• Castellano
  • English
  • Valenciá
Página de inicio de ReDivia
Página de la Generalitat ValenciáPágina de IVIA
Ver ítem 
  •   ReDivia Principal
  • 1.- Investigación
  • 1.1.- Artículos de revista académica
  • Ver ítem
  •   ReDivia Principal
  • 1.- Investigación
  • 1.1.- Artículos de revista académica
  • Ver ítem
JavaScript is disabled for your browser. Some features of this site may not work without it.

Effect of temperature on RNA silencing of a negative-stranded RNA plant virus: Citrus psorosis virus

Exportar
untranslatedRefworks
URI
http://hdl.handle.net/20.500.11939/4686
DOI
10.1111/j.1365-3059.2010.02315.x
Derechos de acceso
openAccess
Metadatos
Mostrar el registro completo del ítem
Autor
Velázquez, KareliaAutoridad IVIA; Renovell, A.; Comellas, M.; Serra, P.; Garcia, M. L.; Pina, José A.Autoridad IVIA; Navarro, LuisAutoridad IVIA; Moreno, PedroAutoridad IVIA; Guerri, José
Fecha
2010
Cita bibliográfica
Velazquez, K., Renovell, A., Comellas, M., Serra, P., Garcia, M.L., Pina, J.A., Navarro, L., Moreno, P., Guerri, J. (2010). Effect of temperature on RNA silencing of a negative-stranded RNA plant virus: Citrus psorosis virus. Plant Pathology, 59(5), 982-990.
Resumen
Citrus psorosis virus (CPsV), genus Ophiovirus, causes a bark scaling disease of citrus. CPsV virions are kinked filaments with three negative-stranded RNA molecules (vRNA) and a 48 kDa coat protein. The effect of temperature on symptom expression, virus accumulation and RNA silencing was examined in sweet orange seedlings (Citrus sinensis) graft-inoculated with three different CPsV isolates and grown in a glasshouse at 26/18 degrees C or 32/26 degrees C (day/night). Most plants kept in the cooler glasshouse showed a shock reaction in the first flush with shoot necrosis, and then moderate to intense chlorotic flecking and spotting in young leaves, whereas plants incubated at 32/26 degrees C did not exhibit shoot necrosis, and young leaf symptoms were milder. Virus titre estimated by ELISA and by northern and dot blot hybridization paralleled symptom intensity, with significantly higher virus accumulation in plants incubated at 26/18 degrees C. The amount of CPsV-derived small RNAs (CPsV-sRNAs) slightly increased at 32/26 degrees C, with the ratio of CPsV-sRNA/vRNA being higher at 32/26 degrees C than at 26/18 degrees C. These results suggest that (i) CPsV infection induces RNA silencing in citrus plants, (ii) symptom intensity is associated with virus accumulation, and (iii) temperature increase enhances the RNA silencing response of citrus plants and decreases virus accumulation.
Colecciones
  • 1.1.- Artículos de revista académica

Listar

Todo ReDiviaComunidades & ColeccionesPor fecha de publicaciónAutoresTítulosMateriasTemasCentros IVIAEsta colecciónPor fecha de publicaciónAutoresTítulosMateriasTemasCentros IVIA

Mi cuenta

AccederRegistro

De interés

Política de Acceso Abierto del IVIAPropiedad intelectual y derechos de autorAutoarchivoPreguntas frecuentes

Indexadores

RecolectaSherpa RomeoDulcinea

Estadísticas

Ver Estadísticas de uso
Creative Commons License

El contenido de este sitio está bajo una licencia Creative Commons - No comercial - Sin Obra Derivada (by-nc-nd), salvo que se indique lo contrario.