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dc.contributor.authorBallester, Alida
dc.contributor.authorCervera, Magdalena
dc.contributor.authorPena, Leandro
dc.date.accessioned2017-06-01T10:10:41Z
dc.date.available2017-06-01T10:10:41Z
dc.date.issued2007
dc.identifier.citationBallE., Alida, Cervera, M., Pena, L. (2007). Efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination. Plant Cell Reports, 26(1), 39-45.
dc.identifier.issn0721-7714
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4661
dc.description.abstractThe presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. In citrus, selection using the selectable marker gene nptII, that confers resistance to the antibiotic kanamycin, is in general very effective. An attractive alternative is offered by the MAT system (Multi-Auto-Transformation), which combines the ipt gene for positive selection with the recombinase system R/RS for removal of marker genes from transgenic cells after transformation. Transformation with a MAT vector has been attempted in two citrus genotypes, Pineapple sweet orange (Citrus sinensis L. Osb.) and Carrizo citrange (C. sinensis L. Osb. x Poncirus trifoliata L. Raf.). Results indicated that the IPT phenotype was clearly distinguishable in sweet orange but not in citrange, and that excision was not always efficient and precise. Nevertheless, the easy visual detection of the IPT phenotype combined with the higher transformation efficiency achieved in sweet orange using this system open interesting perspectives for the generation of marker-free transgenic citrus plants.
dc.language.isoen
dc.titleEfficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormJAN 2007
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1007/s00299-006-0197-3
dc.journal.abbreviatedTitlePlant Cell Rep.
dc.journal.issueNumber1
dc.journal.titlePlant Cell Reports
dc.journal.volumeNumber26
dc.page.final45
dc.page.initial39
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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