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dc.contributor.authorTomás, Cristina
dc.contributor.authorGómez-Fernández, José
dc.contributor.authorGómez-Izquierdo, Emilio
dc.contributor.authorMocé, Eva
dc.contributor.authorDe-Mercado, Eduardo
dc.date.accessioned2017-06-01T10:10:29Z
dc.date.available2017-06-01T10:10:29Z
dc.date.issued2014
dc.identifier.citationTomas, C., Gomez-Fernandez, J., Gomez-Izquierdo, E., Moce, E. & de-Mercado, E. (2014). Addition of CholE.ol-Loaded Cyclodextrins to the Thawing Extender: Effects on Boar Sperm Quality. Reproduction in Domestic Animals, 49(3), 427-432.
dc.identifier.issn0936-6768
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4589
dc.description.abstractThe aim of the present study was to evaluate the effect that the addition of cholesterol‐loaded cyclodextrins (CLC) to the thawing extender has on the quality of frozen‐thawed boar sperm. Pooled semen (n = 5) from three boars was used for the experiments. The semen was cryopreserved with an egg‐yolk‐based extender, it was diluted after thawing in Beltsville thawing solution (BTS) supplemented with different concentrations of CLC (0, 12.5, 25, 50 or 100 mg/500 × 106 sperm), and these samples were incubated at 37°C for 150 min. The following parameters of sperm quality were evaluated 30 and 150 min after incubation: sperm with intact plasma membrane (SIPM; %), sperm with normal acrosomal ridge (NAR; %), total motile sperm (TMS; %), progressively motile sperm (PMS; %) and kinetic parameters. Both SIPM and NAR increased (p < 0.05) when the thawing extender was supplemented with 12.5, 25 and 50 mg CLC/500 × 106 sperm. Nevertheless, motility decreased (p < 0.05) when the concentration of CLC exceeded 12.5 mg CLC/500 × 106 sperm. In conclusion, our results suggest that the supplementation of thawing extenders with CLC improves sperm viability and reduces acrosome damage after freezing/thawing.
dc.language.isoen
dc.titleAddition of Cholesterol-Loaded Cyclodextrins to the Thawing Extender: Effects on Boar Sperm Quality
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormJUN 2014
dc.entidadIVIACentro de Tecnología Animal
dc.identifier.doi10.1111/rda.12291
dc.journal.issueNumber3
dc.journal.titleReproduction in Domestic Animals
dc.journal.volumeNumber49
dc.page.final432
dc.page.initial427
dc.relation.projectIDhttps://onlinelibrary.wiley.com/doi/10.1111/rda.12291
dc.relation.projectIDThis research was supported by project 2010-1282 Plan de Investigación del ITACyL. E. Mocé was supported by funds from Ministerio deCiencia e Innovación from Subprograma Ramón y Cajal co-financedby the European Social Fund (Ref. RYC-2010-06162; Madrid, Spain).
dc.relation.projectIDThis research was supported by project 2010-1282 Plan de Investigación del ITACyL. E. Mocé was supported by funds from Ministerio deCiencia e Innovación from Subprograma Ramón y Cajal co-financedby the European Social Fund (Ref. RYC-2010-06162; Madrid, Spain).
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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