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Effect of a pre-freezing treatment with cholesterol-loaded cyclodextrins on boar sperm longevity, capacitation dynamics, ability to adhere to porcine oviductal epithelial cells in vitro and DNA fragmentation dynamics

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URI
http://hdl.handle.net/20.500.11939/4585
DOI
10.1071/RD12079
Derechos de acceso
openAccess
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Author
Tomás, Cristina; Blanch, Eva; Fazeli, A.; Mocé, Eva
Date
2013
Cita bibliográfica
Tomas, C., Blanch, E., Fazeli, A., Moce, E. (2013). Effect of a pre-freezing treatment with cholE.ol-loaded cyclodextrins on boar sperm longevity, capacitation dynamics, ability to adhere to porcine oviductal epithelial cells in vitro and DNA fragmentation dynamics. Reproduction Fertility and Development, 25(6), 935-946.
Abstract
The aim of this work was to examine how a pre-freezing treatment with cholesterol-loaded cyclodextrins (CLC) affects boar sperm longevity, capacitation dynamics, ability to bind to a porcine telomerase-immortalised oviductal epithelial cell line (TERT-OPEC) in vitro and DNA integrity dynamics after freeze-thawing. Although the samples treated with CLC exhibited lower sperm quality than the control samples (P 0.05) after long-term incubation (26 h at 37 or 16 degrees C). Additionally, the CLC-treated spermatozoa underwent similar capacitation and DNA fragmentation dynamics as the control spermatozoa (P > 0.05). However, CLC-treated spermatozoa were better able to bind to TERT-OPEC in vitro (P < 0.0001). In conclusion, the pre-freezing treatment of boar spermatozoa with CLC enhanced the ability of the spermatozoa to bind to TERT-OPEC in vitro, which could have an effect on the establishment of the sperm reservoir in the ampullary-isthmic junction in vivo. Additionally, frozen-thawed spermatozoa can be stored at 16 degrees C for at least 6 h without a significant observable decline in sperm quality, which could be beneficial for the transport of thawed diluted doses of spermatozoa from the laboratory to the farm.
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