dc.contributor.author | Terol, Javier | |
dc.contributor.author | Ibanez, Victoria | |
dc.contributor.author | Carbonell, Jose | |
dc.contributor.author | Alonso, Roberto | |
dc.contributor.author | Estornell, Leandro H. | |
dc.contributor.author | Licciardello, Concetta | |
dc.contributor.author | Gut, Ivo G. | |
dc.contributor.author | Dopazo, Joaquín | |
dc.contributor.author | Talón, Manuel | |
dc.date.accessioned | 2017-06-01T10:10:28Z | |
dc.date.available | 2017-06-01T10:10:28Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Terol, J., Ibanez, Victoria, Carbonell, J., Alonso, Roberto, Estornell, L. H., Licciardello, Concetta, Gut, Ivo G., Dopazo, Joaqun, Talon, M. (2015). Involvement of a citrus meiotic recombination TTC-repeat motif in the formation of gross deletions generated by ionizing radiation and MULE activation. Bmc Genomics, 16, 69-69. | |
dc.identifier.issn | 1471-2164 | |
dc.identifier.uri | http://hdl.handle.net/20.500.11939/4579 | |
dc.description.abstract | Background: Transposable-element mediated chromosomal rearrangements require the involvement of two transposons and two double-strand breaks (DSB) located in close proximity. In radiobiology, DSB proximity is also a major factor contributing to rearrangements. However, the whole issue of DSB proximity remains virtually unexplored. Results: Based on DNA sequencing analysis we show that the genomes of 2 derived mutations, Arrufatina (sport) and Nero (irradiation), share a similar 2 Mb deletion of chromosome 3. A 7 kb Mutator like element found in Clemenules was present in Arrufatina in inverted orientation flanking the 5' end of the deletion. The Arrufatina Mule displayed "dissimilar" 9-bp target site duplications separated by 2 Mb. Fine-scale single nucleotide variant analyses of the deleted fragments identified a TTC-repeat sequence motif located in the center of the deletion responsible of a meiotic crossover detected in the citrus reference genome. Conclusions: Taken together, this information is compatible with the proposal that in both mutants, the TTC-repeat motif formed a triplex DNA structure generating a loop that brought in close proximity the originally distinct reactive ends. In Arrufatina, the loop brought the Mule ends nearby the 2 distinct insertion target sites and the inverted insertion of the transposable element between these target sites provoked the release of the in-between fragment. This proposal requires the involvement of a unique transposon and sheds light on the unresolved question of how two distinct sites become located in close proximity. These observations confer a crucial role to the TTC-repeats in fundamental plant processes as meiotic recombination and chromosomal rearrangements. | |
dc.language.iso | en | |
dc.title | Involvement of a citrus meiotic recombination TTC-repeat motif in the formation of gross deletions generated by ionizing radiation and MULE activation | |
dc.type | article | |
dc.authorAddress | Instituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), España | es |
dc.date.issuedFreeForm | FEB 13 2015 | |
dc.entidadIVIA | Centro de Genómica | |
dc.identifier.doi | 10.1186/s12864-015-1280-3 | |
dc.journal.abbreviatedTitle | BMC Genomics | |
dc.journal.title | Bmc Genomics | |
dc.journal.volumeNumber | 16 | |
dc.page.final | 69 | |
dc.page.initial | 69 | |
dc.rights.accessRights | openAccess | |
dc.source.type | Impreso | |
dc.type.hasVersion | publishedVersion | |