• Castellano
  • English
  • Valenciá
Página de inicio de ReDivia
Página de la Generalitat ValenciáPágina de IVIA
Ver ítem 
  •   ReDivia Principal
  • 1.- Investigación
  • 1.1.- Artículos de revista académica
  • Ver ítem
  •   ReDivia Principal
  • 1.- Investigación
  • 1.1.- Artículos de revista académica
  • Ver ítem
JavaScript is disabled for your browser. Some features of this site may not work without it.

Involvement of a citrus meiotic recombination TTC-repeat motif in the formation of gross deletions generated by ionizing radiation and MULE activation

Ver/
Open 2015_Terol Involvement.pdf (3.027Mb)
Exportar
untranslatedRefworks
URI
http://hdl.handle.net/20.500.11939/4579
DOI
10.1186/s12864-015-1280-3
Derechos de acceso
openAccess
Metadatos
Mostrar el registro completo del ítem
Autor
Terol, JavierAutoridad IVIA; Ibanez, VictoriaAutoridad IVIA; Carbonell, Jose; Alonso, Roberto; Estornell, Leandro H.; Licciardello, Concetta; Gut, Ivo G.; Dopazo, Joaquín; Talón, ManuelAutoridad IVIA
Fecha
2015
Cita bibliográfica
Terol, J., Ibanez, Victoria, Carbonell, J., Alonso, Roberto, Estornell, L. H., Licciardello, Concetta, Gut, Ivo G., Dopazo, Joaqun, Talon, M. (2015). Involvement of a citrus meiotic recombination TTC-repeat motif in the formation of gross deletions generated by ionizing radiation and MULE activation. Bmc Genomics, 16, 69-69.
Resumen
Background: Transposable-element mediated chromosomal rearrangements require the involvement of two transposons and two double-strand breaks (DSB) located in close proximity. In radiobiology, DSB proximity is also a major factor contributing to rearrangements. However, the whole issue of DSB proximity remains virtually unexplored. Results: Based on DNA sequencing analysis we show that the genomes of 2 derived mutations, Arrufatina (sport) and Nero (irradiation), share a similar 2 Mb deletion of chromosome 3. A 7 kb Mutator like element found in Clemenules was present in Arrufatina in inverted orientation flanking the 5' end of the deletion. The Arrufatina Mule displayed "dissimilar" 9-bp target site duplications separated by 2 Mb. Fine-scale single nucleotide variant analyses of the deleted fragments identified a TTC-repeat sequence motif located in the center of the deletion responsible of a meiotic crossover detected in the citrus reference genome. Conclusions: Taken together, this information is compatible with the proposal that in both mutants, the TTC-repeat motif formed a triplex DNA structure generating a loop that brought in close proximity the originally distinct reactive ends. In Arrufatina, the loop brought the Mule ends nearby the 2 distinct insertion target sites and the inverted insertion of the transposable element between these target sites provoked the release of the in-between fragment. This proposal requires the involvement of a unique transposon and sheds light on the unresolved question of how two distinct sites become located in close proximity. These observations confer a crucial role to the TTC-repeats in fundamental plant processes as meiotic recombination and chromosomal rearrangements.
Colecciones
  • 1.1.- Artículos de revista académica

Listar

Todo ReDiviaComunidades & ColeccionesPor fecha de publicaciónAutoresTítulosMateriasTemasCentros IVIAEsta colecciónPor fecha de publicaciónAutoresTítulosMateriasTemasCentros IVIA

Mi cuenta

AccederRegistro

De interés

Política de Acceso Abierto del IVIAPropiedad intelectual y derechos de autorAutoarchivoPreguntas frecuentes

Indexadores

RecolectaSherpa RomeoDulcinea

Estadísticas

Ver Estadísticas de uso
Creative Commons License

El contenido de este sitio está bajo una licencia Creative Commons - No comercial - Sin Obra Derivada (by-nc-nd), salvo que se indique lo contrario.