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dc.contributor.authorTeresani, Gabriela
dc.contributor.authorBertolini, Edson
dc.contributor.authorAlfaro-Fernández, Ana
dc.contributor.authorMartínez, M. Carmen
dc.contributor.authorOssamu Tanaka, Francisco Andre
dc.contributor.authorKitajima, Elliot W.
dc.contributor.authorRoselló, Montserrat
dc.contributor.authorSanjuan, Susana
dc.contributor.authorCarlos Ferrandiz, Juan
dc.contributor.authorLópez, María M.
dc.contributor.authorCambra, Mariano
dc.contributor.authorFont-San-Ambrosio, Isabel
dc.identifier.citationTeresani, Gabriela R., Bertolini, E., Alfaro-Fernandez, Ana, Martinez, C., Ossamu Tanaka, F. Andre, Kitajima, Elliot W., Rosello, Montserrat, SanJ., S., C. Ferrandiz, J., Lopez, M.M., Cambra, M., I. Font, M. (2014). Association of 'Candidatus Liberibacter solanacearum' with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection. Phytopathology, 104(8), 804-811.
dc.description.abstractA new symptomatology was observed in celery (Apium graveolens) in Villena, Spain in 2008. Symptomatology included an abnormal amount of shoots per plant and curled stems. These vegetative disorders were associated with ‘Candidatus Liberibacter solanacearum’ and not with phytoplasmas. Samples from plant sap were immobilized on membranes based on the spot procedure and tested using a newly developed real-time polymerase chain reaction assay to detect ‘Ca. L. solanacearum’. Then, a test kit was developed and validated by intralaboratory assays with an accuracy of 100%. Bacterial-like cells with typical morphology of ‘Ca. Liberibacter’ were observed using electron microscopy in celery plant tissues. A fifth haplotype of ‘Ca. L. solanacearum’, named E, was identified in celery and in carrot after analyzing partial sequences of 16S and 50S ribosomal RNA genes. From our results, celery (family Apiaceae) can be listed as a new natural host of this emerging bacterium.
dc.titleAssociation of 'Candidatus Liberibacter solanacearum' with a Vegetative Disorder of Celery in Spain and Development of a Real-Time PCR Method for Its Detection
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes 2014
dc.entidadIVIACentro de Protección Vegetal y Biotecnología

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