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dc.contributor.authorScuderi, Giuseppe
dc.contributor.authorGolmohammadi, Morteza
dc.contributor.authorCubero, Jaime
dc.contributor.authorLópez, María M.
dc.contributor.authorCirvilleri, G.
dc.contributor.authorLlop, Pablo
dc.date.accessioned2017-06-01T10:10:18Z
dc.date.available2017-06-01T10:10:18Z
dc.date.issued2010
dc.identifier.citationScuderi, G., Golmohammadi, M., Cubero, J., Lopez, M. M., Cirvilleri, G. & Llop, P. (2010). Development of a simplified NASBA protocol for detecting viable cells of the citrus pathogen Xanthomonas citri subsp citri under different treatments. Plant Pathology, 59(4), 764-772.
dc.identifier.issn0032-0862
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4506
dc.description.abstractNucleic acid sequence based amplification (NASBA) is a method of amplifying RNA, for the detection of RNA viruses and human pathogenic bacteria. Recently, NASBA has also been employed for the detection of plant diseases caused by viruses and quarantine bacteria. A major citrus pathogen, Xanthomonas citri subsp. citri (Xcc), causal agent of citrus bacterial canker, is being studied in depth due to its economic importance, with recent focus concentrating on its viability and survival under different stress conditions and control treatments. In this work, a NASBA protocol using primers for gumD mRNA has been developed to assess the viability of this pathogen under different bacteriocidal treatments. This method is rapid, specific and sensitive, and is able to detect viable bacterial cells, using a hybridization device which allows the visualization of the results in only 30 min. The usefulness of the method has been confirmed with bacterial suspensions subjected to different heat treatments and to sodium orthophenylphenate.
dc.language.isoen
dc.titleDevelopment of a simplified NASBA protocol for detecting viable cells of the citrus pathogen Xanthomonas citri subsp citri under different treatments
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.authorAddressllop_pab@gva.es
dc.date.issuedFreeFormAUG 2010
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1111/j.1365-3059.2010.02305.x
dc.identifier.urlhttps://bsppjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/j.1365-3059.2010.02305.x
dc.journal.abbreviatedTitlePlant Pathol.
dc.journal.issueNumber4
dc.journal.titlePlant Pathology
dc.journal.volumeNumber59
dc.page.final772
dc.page.initial764
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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