Effect of different extenders and washing of seminal plasma on buck semen storage at 5 degrees C

Abstract

In this research, we compared the effect of three extenders for buck semen conservation; skimmed Milk (M), sodium Citrate (C) and a Tris-based diluent (T) and the washing of semen (removal of seminal plasma) on the in vitro viability of Murciano-Granadina goat spermatozoa stored at 5 degrees C for 72 h. Motility, acrosome integrity and HOS test were evaluated to assess in vitro sperm viability. Milk diluent provided higher in vitro viability of spermatozoa than semen diluted in T during storage at 5 degrees C. Motility in semen diluted in citrate and milk extenders was improved when semen was washed previously. In milk extender, membrane integrity (HOST) was also improved with the washing of semen. In conclusion, removal of seminal plasma could be necessary for successful chilled conservation of buck semen at 5 degrees C when M or C based diluents is used. Milk media and washing of seminal plasma appears to be a successful method to prolong the viability of Murciano-Granadina goat semen stored at 5 degrees C. The latest results must be confirmed in field assays.