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dc.contributor.authorRuiz-Ruiz, Susana
dc.contributor.authorNavarro, Beatriz
dc.contributor.authorGisel, Andreas
dc.contributor.authorPena, Leandro
dc.contributor.authorNavarro, Luis
dc.contributor.authorMoreno, Pedro
dc.contributor.authorDi Serio, Francesco
dc.contributor.authorFlores, Ricardo
dc.date.accessioned2017-06-01T10:10:11Z
dc.date.available2017-06-01T10:10:11Z
dc.date.issued2011
dc.identifier.citationRuiz-Ruiz, S., Navarro, Beatriz, Gisel, Andreas, Pena, L., Navarro, L., Moreno, P., Di Serio, Francesco, Flores, R. (2011). Citrus tristeza virus infection induces the accumulation of viral small RNAs (21-24-nt), mapping preferentially at the 3'-terminal region of the genomic RNA and affects the host small RNA profile. Plant Molecular Biology, 75(6), 607-619.
dc.identifier.issn0167-4412
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4457
dc.description.abstractTo get an insight into the host RNA silencing defense induced by Citrus tristeza virus (CTV) and into the counter defensive reaction mediated by its three silencing suppressors (p25, p20 and p23), we have examined by deep sequencing (Solexa-Illumina) the small RNAs (sRNAs) in three virus-host combinations. Our data show that CTV sRNAs: (i) represent more than 50% of the total sRNAs in Mexican lime and sweet orange (where CTV reaches relatively high titers), but only 3.5% in sour orange (where the CTV titer is significantly lower), (ii) are predominantly of 21-22-nt, with a biased distribution of their 5' nucleotide and with those of (+) polarity accumulating in a moderate excess, and (iii) derive from essentially all the CTV genome (ca. 20 kb), as revealed by its complete reconstruction from viral sRNA contigs, but adopt an asymmetric distribution with a prominent hotspot covering approximately the 3'-terminal 2,500 nt. These results suggest that the citrus homologues of Dicer-like (DCL) 4 and 2 most likely mediate the genesis of the 21 and 22 nt CTV sRNAs, respectively, and show that both ribonucleases act not only on the genomic RNA but also on the 3' co-terminal subgenomic RNAs and, particularly, on their double-stranded forms. The plant sRNA profile, very similar and dominated by the 24-nt sRNAs in the three mock-inoculated controls, was minimally affected by CTV infection in sour orange, but exhibited a significant reduction of the 24-nt sRNAs in Mexican lime and sweet orange. We have also identified novel citrus miRNAs and determined how CTV influences their accumulation.
dc.language.isoen
dc.titleCitrus tristeza virus infection induces the accumulation of viral small RNAs (21-24-nt) mapping preferentially at the 3'-terminal region of the genomic RNA and affects the host small RNA profile
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormAPR
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1007/s11103-011-9754-4
dc.journal.abbreviatedTitlePlant Mol.Biol.
dc.journal.issueNumber6
dc.journal.titlePlant Molecular Biology
dc.journal.volumeNumber75
dc.page.final619
dc.page.initial607
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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