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dc.contributor.authorRubio, Luis
dc.contributor.authorJanssen, Dirk
dc.contributor.authorCuadrado, I. M.
dc.contributor.authorMoreno, Pedro
dc.contributor.authorGuerri, José
dc.date.accessioned2017-06-01T10:10:09Z
dc.date.available2017-06-01T10:10:09Z
dc.date.issued2003
dc.identifier.citationRubio, L., Janssen, D., Cuadrado, I. M., Moreno, P., Guerri, J. (2003). Rapid detection of cucumber vein yellowing virus by tissue-print hybridisation with digoxigenin-labelled cDNA probes. Journal of virological methods, 114(1), 105-107.
dc.identifier.issn0166-0934
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4447
dc.description.abstractHybridisation of tissue prints with nonradioactive cDNA probes was developed to detect cucumber vein yellowing virus (CVYV) in cucurbit plants. Results showed irregular distribution of the virus within cucumber, zucchini or melon plants without defined tropism for a specific tissue. Therefore, reliable diagnosis of CVYV requires analysis of tissue prints from at least five different plant sites. This detection procedure allows rapid analysis of large numbers of plants and it can be useful for epidemiological studies of CVYV and to control virus spread via eradication of early foci.
dc.language.isoen
dc.titleRapid detection of cucumber vein yellowing virus by tissue-print hybridisation with digoxigenin-labelled cDNA probes
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormDEC 2003
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1016/j.jviromet.2003.08.008
dc.journal.abbreviatedTitleJ.Virol.Methods
dc.journal.issueNumber1
dc.journal.titleJournal of virological methods
dc.journal.volumeNumber114
dc.page.final107
dc.page.initial105
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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