Annotation and overview of the Pseudomonas savastanoi pv. savastanoi NCPPB 3335 draft genome reveals the virulence gene complement of a tumour-inducing pathogen of woody hosts

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URI
http://hdl.handle.net/20.500.11939/4435
DOI
10.1111/j.1462-2920.2010.02207.x
Derechos de acceso
openAccess
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Author
Rodriguez-Palenzuela, Pablo; Matas, Isabel M.; Murillo, Jesús; Lopez-Solanilla, Emilia; Bardaji, Leire; Perez-Martinez, Isabel; Rodriguez-Moskera, Martin E.; Penyalver, Ramón; López, María M.; Quesada, Jose M.; Biehl, Bryan S.; Perna, Nicole T.; Glasner, Jeremy D.; Cabot, Eric L.; Neeno-Eckwall, Eric; Ramos, Cayo
Date
2010
Cita bibliográfica
Rodríguez‐Palenzuela, P., Matas, I. M., Murillo, J., López‐Solanilla, E., Bardaji, L., Pérez‐Martínez, I. et al. (2010). Annotation and overview of the Pseudomonas savastanoi pv. savastanoi NCPPB 3335 draft genome reveals the virulence gene complement of a tumour‐inducing pathogen of woody hosts. Environmental microbiology, 12(6), 1604-1620.
Abstract
Pseudomonas savastanoi pv. savastanoi is a tumour-inducing pathogen of Olea europaea L. causing oliveknot disease. Bioinformatic analysis of the draftgenome sequence of strain NCPPB 3335, whichencodes 5232 predicted coding genes on a totallength of 5856 998 bp and a 57.12% G + C, revealed alarge degree of conservation with Pseudomonassyringae pv. phaseolicola 1448A and P. syringae pv.tabaci 11528. However, NCPPB 3335 contains twelvevariable genomic regions, which are absent in all pre-viously sequenced P. syringae strains. Various fea-tures that could contribute to the ability of this strainto survive in a woody host were identified, includingbroad catabolic and transport capabilities for degrad-ing plant-derived aromatic compounds, the duplica-tion of sequences related to the biosynthesis of thephytohormone indoleacetic acid (iaaM, iaaH) and itsamino acid conjugate indoleacetic acid-lysine (iaaLgene), and the repertoire of strain-specific putativetype III secretion system effectors. Access to thisseventh genome sequence belonging to the ‘P. syrin-gae complex’ allowed us to identify 73 predictedcoding genes that are NCPPB 3335-specific. Resultsshown here provide the basis for detailed functionalanalysis of a tumour-inducing pathogen of woodyhosts and for the study of specific adaptations of a P.savastanoi pathovar.
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