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dc.contributor.authorRodriguez, Ana
dc.contributor.authorShimada, Takehiko
dc.contributor.authorCervera, Magdalena
dc.contributor.authorAlquezar, Berta
dc.contributor.authorGadea, Jose
dc.contributor.authorGómez-Cadenas, Aurelio
dc.contributor.authorDe-Ollas, Carlos J.
dc.contributor.authorRodrigo, María J.
dc.contributor.authorZacarias, Lorenzo
dc.contributor.authorPena, Leandro
dc.identifier.citationRodriguez, A., Shimada, Takehiko, Cervera, M., Alquezar, B., Gadea, J., Gomez-Cadenas, Aurelio, J. De Ollas, C., Jesus Rodrigo, M., Zacarias, L., Pena, L. (2014). Terpene Down-Regulation Triggers Defense Responses in Transgenic Orange Leading to Resistance against Fungal Pathogens. Plant Physiology, 164(1), 321-339.
dc.description.abstractTerpenoid volatiles are isoprene compounds that are emitted by plants to communicate with the environment. In addition to their function in repelling herbivores and attracting carnivorous predators in green tissues, the presumed primary function of terpenoid volatiles released from mature fruits is the attraction of seed-dispersing animals. Mature oranges (Citrus sinensis) primarily accumulate terpenes in peel oil glands, with D-limonene accounting for approximately 97% of the total volatile terpenes. In a previous report, we showed that down-regulation of a D-limonene synthase gene alters monoterpene levels in orange antisense (AS) fruits, leading to resistance against Penicillium digitatum infection. A global gene expression analysis of AS versus empty vector (EV) transgenic fruits revealed that the down-regulation of D-limonene up-regulated genes involved in the innate immune response. Basal levels of jasmonic acid were substantially higher in the EV compared with AS oranges. Upon fungal challenge, salicylic acid levels were triggered in EV samples, while jasmonic acid metabolism and signaling were drastically increased in AS orange peels. In nature, D-limonene levels increase in orange fruit once the seeds are fully viable. The inverse correlation between the increase in D-limonene content and the decrease in the defense response suggests that D-limonene promotes infection by microorganisms that are likely involved in facilitating access to the pulp for seed-dispersing frugivores.
dc.titleTerpene Down-Regulation Triggers Defense Responses in Transgenic Orange Leading to Resistance against Fungal Pathogens
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes 2014
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.journal.abbreviatedTitlePlant Physiol.
dc.journal.titlePlant Physiology

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