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Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids

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URI
http://hdl.handle.net/20.500.11939/4354
DOI
10.1023/A:1026433405701
Derechos de acceso
openAccess
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Author
Perez, Rosa; Mas, O.; Navarro, LuisAutoridad IVIA; Durán-Vila, NúriaAutoridad IVIA
Date
1999
Cita bibliográfica
Perez, R., Mas, O., Navarro, L.& Duran-Vila, N. (1999). Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids. Plant Cell Tissue and Organ Culture, 55(1), 71-74.
Abstract
Assays were performed to obtain embryogenic callus lines from nine mandarin and mandarin hybrid cultivars by in vitro culture of ovules collected from immature fruits six weeks after anthesis. All cultivars produced embryos and loose friable callus. The small proliferations of nucellar callus from cultured ovules were suitable to recover embryogenic callus lines by periodical subculturing to fresh medium. The embryogenic callus cultures were subjected to cryoprotection with 10% (v/v) (DMSO), freezing by slow cooling, storage in liquid nitrogen and thawing by fast warming. Whole plants from these cryopreserved cultures were recovered through embryogenesis.
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