Show simple item record

dc.contributor.authorOomen, Ronald J. F. J.
dc.contributor.authorBenito, Begona
dc.contributor.authorSentenac, Herve
dc.contributor.authorRodriguez-Navarro, Alonso
dc.contributor.authorTalón, Manuel
dc.contributor.authorVery, Anne-Alienor
dc.contributor.authorDomingo, Concha
dc.date.accessioned2017-06-01T10:09:43Z
dc.date.available2017-06-01T10:09:43Z
dc.date.issued2012
dc.identifier.citationOomen, Ronald J. F. J., Benito, Begona, Sentenac, Herve, Rodriguez-Navarro, Alonso, Talón, M., Very, Anne-Alienor, Domingo, C. (2012). HKT2;2/1, a K+-permeable transporter identified in a salt-tolerant rice cultivar through surveys of natural genetic polymorphism. Plant Journal, 71(5), 750-762.
dc.identifier.issn0960-7412
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4265
dc.description.abstractWe have investigated OsHKT2;1 natural variation in a collection of 49 cultivars with different levels of salt tolerance and geographical origins. The effect of identified polymorphism on OsHKT2;1 activity was analysed through heterologous expression of variants in Xenopus oocytes. OsHKT2;1 appeared to be a highly conserved protein with only five possible amino acid substitutions that have no substantial effect on functional properties. Our study, however, also identified a new HKT isoform, No-OsHKT2;2/1 in Nona Bokra, a highly salt-tolerant cultivar. No-OsHKT2;2/1 probably originated from a deletion in chromosome 6, producing a chimeric gene. Its 5' region corresponds to that of OsHKT2;2, whose full-length sequence is not present in Nipponbare but has been identified in Pokkali, a salt-tolerant rice cultivar. Its 3' region corresponds to that of OsHKT2;1. No-OsHKT2;2/1 is essentially expressed in roots and displays a significant level of expression at high Na+ concentrations, in contrast to OsHKT2;1. Expressed in Xenopus oocytes or in Saccharomyces cerevisiae, No-OsHKT2;2/1 exhibited a strong permeability to Na+ and K+, even at high external Na+ concentrations, like OsHKT2;2, and in contrast to OsHKT2;1. Our results suggest that No-OsHKT2;2/1 can contribute to Nona Bokra salt tolerance by enabling root K+ uptake under saline conditions.
dc.language.isoen
dc.titleHKT2;2/1, a K+-permeable transporter identified in a salt-tolerant rice cultivar through surveys of natural genetic polymorphism
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormSEP 2012
dc.entidadIVIACentro de Genómica
dc.identifier.doi10.1111/j.1365-313X.2012.05031.x
dc.identifier.urlhttps://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-313X.2012.05031.x
dc.journal.issueNumber5
dc.journal.titlePlant Journal
dc.journal.volumeNumber71
dc.page.final762
dc.page.initial750
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionacceptedVersion


Files in this item

This item appears in the following Collection(s)

Show simple item record