dc.contributor.author | Olmos, Antonio | |
dc.contributor.author | Cambra, Mariano | |
dc.contributor.author | Dasi, M. A. | |
dc.contributor.author | Candresse, Thierry | |
dc.contributor.author | Esteban, Olga | |
dc.contributor.author | Gorris, María T. | |
dc.contributor.author | Asensio, M. | |
dc.date.accessioned | 2017-06-01T10:09:42Z | |
dc.date.available | 2017-06-01T10:09:42Z | |
dc.date.issued | 1997 | |
dc.identifier.citation | Olmos, A., Cambra, M., Dasi, M.A., Candresse, T., Esteban, O., Gorris, M. T., Asensio, M. (1997). Simultaneous detection and typing of plum pox potyvirus (PPV), isolates by heminested-PCR and PCR-ELISA. Journal of virological methods, 68(2), 127-137. | |
dc.identifier.issn | 0166-0934 | |
dc.identifier.uri | http://hdl.handle.net/20.500.11939/4259 | |
dc.description.abstract | Two techniques for simultaneous detection and typing of plum pox potyvirus (PPV) isolates belonging to the D or M serotypes, heminested PCR (H-PCR) and PCR-ELISA, have been developed. Ten PPV isolates typed using PPV-D and PPV-M specific monoclonal antibodies by ELISA-DASI were used to validate these two methods. The results obtained show a complete coincidence of the nucleic acid-based techniques with the serological data. When serial dilutions of infected plant extracts were assayed, H-PCR and PCR-ELISA were found to be 100 rimes more sensitive than the more conventional immunocapture-PCR (IC-PCR) assay. Testing of 228 PPV-infected fruit tree samples coming from different hosts and locations indicated that so fat-only PPV type D appears to be present in Spain and in Chile. Coupled with print-capture sample preparation (Olmos et al., Nucl. Acids Res. 24, 2192-2193, 1996) the increased sensitivity provided by heminested-PCR allowed the detection of PPV targets of D and M types, in wingless individuals of the aphid vector Aphis gossypii. (C) 1997 Elsevier Science Ireland Ltd. | |
dc.language.iso | en | |
dc.title | Simultaneous detection and typing of plum pox potyvirus (PPV) isolates by heminested-PCR and PCR-ELISA | |
dc.type | article | |
dc.authorAddress | Instituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), España | es |
dc.date.issuedFreeForm | NOV 1997 | |
dc.entidadIVIA | Centro de Protección Vegetal y Biotecnología | |
dc.identifier.doi | 10.1016/S0166-0934(97)00120-1 | |
dc.journal.abbreviatedTitle | J.Virol.Methods | |
dc.journal.issueNumber | 2 | |
dc.journal.title | Journal of virological methods | |
dc.journal.volumeNumber | 68 | |
dc.page.final | 137 | |
dc.page.initial | 127 | |
dc.rights.accessRights | openAccess | |
dc.source.type | Impreso | |