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dc.contributor.authorOllitrault, Patrick
dc.contributor.authorTerol, Javier
dc.contributor.authorChen, Chunxian
dc.contributor.authorFederici, Claire T.
dc.contributor.authorLotfy, Samia
dc.contributor.authorHippolyte, Isabelle
dc.contributor.authorOllitrault, Frederique
dc.contributor.authorBerard, Aurelie
dc.contributor.authorChauveau, Aurelie
dc.contributor.authorCuenca, José
dc.contributor.authorCostantino, Gilles
dc.contributor.authorKacar, Yildiz Aka
dc.contributor.authorMu, Lisa
dc.contributor.authorGarcía-Lor, Andrés
dc.contributor.authorFroelicher, Yann
dc.contributor.authorAleza, Pablo
dc.contributor.authorBoland, Anne
dc.contributor.authorBillot, Claire
dc.contributor.authorNavarro, Luis
dc.contributor.authorLuro, Francois
dc.contributor.authorRoose, Mikeal L.
dc.contributor.authorGmitter Jr., Frederick G.
dc.contributor.authorTalón, Manuel
dc.contributor.authorBrunel, Dominique
dc.date.accessioned2017-06-01T10:09:40Z
dc.date.available2017-06-01T10:09:40Z
dc.date.issued2012
dc.identifier.citationOllitrault, P., Terol, J., Chen, C., Federici, C. T., Lotfy, S., Hippolyte, I., ... & Costantino, G. (2012). A reference genetic map of C. clementina hort. ex Tan.; citrus evolution inferences from comparative mapping. BMC genomics, 13(1), 593.
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4245
dc.description.abstractBackground: Most modern citrus cultivars have an interspecific origin. As a foundational step towards deciphering the interspecific genome structures, a reference whole genome sequence was produced by the International Citrus Genome Consortium from a haploid derived from Clementine mandarin. The availability of a saturated genetic map of Clementine was identified as an essential prerequisite to assist the whole genome sequence assembly. Clementine is believed to be a 'Mediterranean' mandarin x sweet orange hybrid, and sweet orange likely arose from interspecific hybridizations between mandarin and pummelo gene pools. The primary goals of the present study were to establish a Clementine reference map using codominant markers, and to perform comparative mapping of pummelo, sweet orange, and Clementine. Results: Five parental genetic maps were established from three segregating populations, which were genotyped with Single Nucleotide Polymorphism (SNP), Simple Sequence Repeats (SSR) and Insertion-Deletion (Indel) markers. An initial medium density reference map (961 markers for 1084.1 cM) of the Clementine was established by combining male and female Clementine segregation data. This Clementine map was compared with two pummelo maps and a sweet orange map. The linear order of markers was highly conserved in the different species. However, significant differences in map size were observed, which suggests a variation in the recombination rates. Skewed segregations were much higher in the male than female Clementine mapping data. The mapping data confirmed that Clementine arose from hybridization between 'Mediterranean' mandarin and sweet orange. The results identified nine recombination break points for the sweet orange gamete that contributed to the Clementine genome. Conclusions: A reference genetic map of citrus, used to facilitate the chromosome assembly of the first citrus reference genome sequence, was established. The high conservation of marker order observed at the interspecific level should allow reasonable inferences of most citrus genome sequences by mapping next-generation sequencing (NGS) data in the reference genome sequence. The genome of the haploid Clementine used to establish the citrus reference genome sequence appears to have been inherited primarily from the 'Mediterranean' mandarin. The high frequency of skewed allelic segregations in the male Clementine data underline the probable extent of deviation from Mendelian segregation for characters controlled by heterozygous loci in male parents.
dc.language.isoen
dc.titleA reference genetic map of C. clementina hort. ex Tan.; citrus evolution inferences from comparative mappingen
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormNOV 5 2012
dc.entidadIVIACentro de Citricultura y Producción Vegetal
dc.entidadIVIACentro de Genómica
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1186/1471-2164-13-593
dc.journal.abbreviatedTitleBMC Genomics
dc.journal.titleBmc Genomics
dc.journal.volumeNumber13
dc.page.final593
dc.page.initial593
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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