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dc.contributor.authorHerrera-Vásquez, José A.
dc.contributor.authorRubio, Luis
dc.contributor.authorAlfaro-Fernández, Ana
dc.contributor.authorDebreczeni, Diana E.
dc.contributor.authorFont-San-Ambrosio, Isabel
dc.contributor.authorFalk, Bryce W.
dc.contributor.authorFerriol, Inmaculada
dc.identifier.citationAngel Herrera-Vasquez, J., Rubio, L., Alfaro-Fernandez, Ana, Elvira Debreczeni, Diana, Font-San-Ambrosio, I., Falk, Bryce W., Ferriol, I. (2015). Detection and absolute quantitation of Tomato torrado virus (ToTV), by real time RT-PCR. Journal of virological methods, 221, 90-94.
dc.description.abstractTomato torrado virus (ToTV) causes serious damage to the tomato industry and significant economic losses. A quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR) method using primers and a specific TaqMan (R) MGB probe for ToTV was developed for sensitive detection and quantitation of different ToTV isolates. A standard curve using RNA transcripts enabled absolute quantitation, with a dynamic range from 10(4) to 10(10) ToTV RNA copies/ng of total RNA. The specificity of the RT-qPCR was tested with twenty-three ToTV isolates from tomato (Solanum lycopersicum L), and black nightshade (Solanum nigrum L) collected in Spain, Australia, Hungary and France, which covered the genetic variation range of this virus. This new RT-qPCR assay enables a reproducible, sensitive and specific detection and quantitation of ToTV, which can be a valuable tool in disease management programs and epidemiological studies.
dc.titleDetection and absolute quantitation of Tomato torrado virus (ToTV) by real time RT-PCR
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes 1 2015
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.journal.titleJournal of virological methods

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