Proteomic analysis of "Moncada" mandarin leaves with contrasting fruit load
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AuthorMunoz-Fambuena, Natalia; Mesejo, Carlos; Agustí, Manuel; Tarraga, Susana; Iglesias, Domingo J.; Primo-Millo, Eduardo; González-Mas, María C.
Cita bibliográficaMunoz-Fambuena, N., Mesejo, C., Agusti, M., Tarraga, S., Iglesias, D.J., Primo-Millo, E., Gonzalez-Mas, M.C. (2013). Proteomic analysis of "Moncada" mandarin leaves with contrasting fruit load. Plant Physiology and Biochemistry, 62, 95-106.
A proteomic approach was used to know more about the molecular mechanism related to Citrus alternate bearing. To this end, we researched protein expression differences between on-crop and off-crop “Moncada” [Clementine ‘Oroval’ (Citrus clementina Hort ex Tanaka) x ‘Kara’ mandarin (Citrus unshiu Marc. x Citrus nobilis Lou.)] mandarin leaves. This variety usually shows a remarkable behaviour in alternate production. Samples were collected in the period during which the fruit affect flowering induction. From 2D DIGE gel, 110 spots were isolated: 43 showed increased expression in the off-crop samples compared to on-crop samples, while 67 showed increased expression in the on-crop samples against off-crop samples. These spots were identified by MALDI-MS or LC-MS–MS. According to the up-expressed proteins in off-crop leaves such as proteins related to nutrient reservoir activity or to the pentose phosphate pathway, the primary metabolism was more active in off-crop trees than in on-crop trees. In contrast, the proteins up-expressed in on-crop samples such as catalase were related to the oxidoreductase activity and, therefore, the redox state seemed different for off-crop and for on-crop leaves. Other proteins with unknown functions were isolated, which could be also related to the alternate bearing and to the flowering induction.