T1 Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest
A1 Ferguson, Kim B.
A1 Visser, Sander
A1 Daliková, M.
A1 Provazniková, I.
A1 Urbaneja, Alberto
A1 Pérez-Hedo, Meritxell
A1 Marec, F.
A1 Werren, John H.
A1 Zwaan, Bas J.
A1 Pannebakker, Bart A.
A1 Verhulst, Eveline C.
K1 Hemiptera
K1 linked-read
K1 biocontrol
K1 H10  Pests of plants
AB Nesidiocoris tenuis (Reuter) is an efficient predatory biologicalcontrol agent used throughout theMediterraneanBasin in tomato crops but regarded as a pest in northernEuropean countries. Fromthe familyMiridae, it is an economicallyimportant insect yet very little is known interms of genetic information and no genomic or transcriptomicstudies have been published. Here, we usea linked-read sequencing strategy on a single femaleN. tenuis. From this, we assembled the 355 Mbp genomeand delivered an ab initio, homology-based andevidence-based annotation. Along the way, the bacterial“contamination” was removed from the assembly. Inaddition, bacterial lateral gene transfer (LGT) candidateswere detected in the N. tenuis genome. The completegene set is composed of 24 688 genes; the associatedproteins were compared to other hemipterans (Cimexlectularis, Halyomorpha halys and Acyrthosiphonpisum).We visualized the genome using various cytogenetictechniques, such as karyotyping, CGH and GISH,indicating a karyotype of 2n = 32. Additional analysesinclude the localization of 18S rDNA and unique satelliteprobes as well as pooled sequencing to assess nucleotidediversity and neutrality of the commercial population.This is one of the first mirid genomes to bereleased and the first of a mirid biological control agent
PB Wiley
YR 2021
FD 2021
LK http://hdl.handle.net/20.500.11939/7007
UL http://hdl.handle.net/20.500.11939/7007
LA en
NO Ferguson, K.B., Visser, S., Dalíková, M., Provazníková, I., Urbaneja, A., Pérez‐Hedo, M., Marec, F., Werren, J.H., Zwaan, B.J., Pannebakker, B.A. and Verhulst, E.C. (2021), Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest. Insect Mol Biol.
DS MINDS@UW
RD May 16, 2022