RT article
T1 Effect of dsRNA on growth rate and reproductive potential of Monosporascus cannonballus
A1 Armengol, Josep
A1 Alaniz, Sandra
A1 Vicent, Antonio
A1 Beltran, Roberto
A1 Abad-Campos, Paloma
A1 Perez-Sierra, Ana
A1 Garcia-Jimenez, Jose
A1 Ben Salem, Ibtissem
A1 Souli, Mounira
A1 Boughalleb, Naima
AB The effect of double stranded RNA (dsRNA) infection on growth rate and the reproductive potential of Monosporascus cannonballus was studied in 21 isolates collected in cucurbit growing areas of Spain and Tunisia. The isolates were incubated on potato dextrose agar (PDA) under different conditions of temperature, pH, and water potential (Psi(s)). They showed optimal growth temperatures over the range of 27-34 degrees C and perithecia formation was obtained mainly at 25 and 30 degrees C, although some isolates were able to produce perithecia at 35 degrees C. All isolates were able to produce perithecia in a broad range of pHs (4-8). Regarding the effect of Psi(s), the isolates were more tolerant to grow on KCl than on NaCl. For each solute, radial growth decreased progressively as Psi(s) decreased and was severely limited at -5.0 to -6.0 MPa. Perithecia formation was highest at -0.5 MPa, decreased at -1.0 MPa and occurred just in some isolates at -2.0 MPa. Nine of the M. cannonballus isolates harboured dsRNA with 2-6 bands each and a size range of 1.9-18.0 Kb. Phenotypical data were subjected to multivariate factorial analysis. Most of the isolates clustered in two groups corresponding with the presence/absence of dsRNA elements. Isolates without detectable dsRNA produced more perithecia. However, isolates with dsRNA produced lower number of perithecia depending on the pH, Psi(s) or solute used. These results improve our understanding of the behaviour and growth of this pathogen in soil, and can be useful to implement effective disease control. (C) 2011 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
SN 1878-6146
YR 2011
FD 2011
LK http://hdl.handle.net/20.500.11939/4439
UL http://hdl.handle.net/20.500.11939/4439
LA en
NO Armengol, J.p, Alaniz, Sandra, Vicent, A., Beltran, Roberto, Abad-Campos, P., Perez-Sierra, Ana, Garcia-Jimenez, J., Ben Salem, Ibtissem, Souli, Mounira, Boughalleb, Naima (2011). Effect of dsRNA on growth rate and reproductive potential of Monosporascus cannonballus. Fungal Biology, 115(3), 236-244.
DS MINDS@UW
RD Jan 27, 2021