RT conferenceObject
T1 Applications of biotechnology to citrus improvement in Spain
A1 Navarro, Luis
A1 Olivares-Fuster, Oscar
A1 Juárez, José
A1 Aleza, Pablo
A1 Pina, José A.
A1 Ballester-Olmos, J. F.
A1 Cervera, Magdalena
A1 Fagoaga, Carmen
A1 Durán-Vila, Núria
A1 Pena, Leandro
A2 AlbrigoL. G.
A2 Galán SaucoVictor
AB Citrus are subject to many biotic and abiotic stresses and markets are continuously requiring fruits of higher quality. These pose important problems in most citrus growing areas that can only be solved with the establishment of citrus improvement programs to recover new and healthy genotypes to be used as rootstocks and varieties. Success of traditional citrus breeding strategies is limited by the peculiar genetic and reproductive characteristics of citrus. Biotechnology offers different approaches that can overcome many of these limitations. In this paper the following technologies used in Spain are reviewed: a) Shoot tip grafting in vitro. It is used worldwide to recover plants free of all known citrus pathogens, and already hundreds of millions of trees originated in this technique have been planted in the field; b) Embryo rescue. It is used in large programs to recover triploid seedless hybrid varieties from aborted seeds from 2n x 4n and 2n x 2n crosses; c) Protoplast fusion. It allows the production of allotetraploid hybrids among sexually compatible and incompatible parents to be used as rootstocks or as parentals for interploid breeding. Fusions between diploid and haploid cell lines are also used to produce triploid hybrids. d) Genetic transformation. Efficient protocols for transformation of several genotypes have been developed and introduction in citrus of genes of potential agronomical interest has been accomplished.
SN 0567-7572; 90-6605-587-1
YR 2004
FD 2004
LK http://hdl.handle.net/20.500.11939/4212
UL http://hdl.handle.net/20.500.11939/4212
LA en
NO Navarro, L., Olivares-Fuster, O., Juárez, J., Aleza, P., Pina, J.A., Ballester-Olmos, J.F., et al. (2004). Applications of biotechnology to citrus improvement in Spain. Acta Hortic. 632, 221-234.
DS MINDS@UW
RD May 18, 2022