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dc.contributor.authorPrimo-Capella, Amparo
dc.contributor.authorMartínez-Cuenca, Mary-Rus
dc.contributor.authorForner-Giner, María A.
dc.date.accessioned2022-01-03T19:07:34Z
dc.date.available2022-01-03T19:07:34Z
dc.date.issued2021es
dc.identifier.citationPrimo-Capella, A., Martinez-Cuenca, M. R. & Forner-Giner, M. A. (2021). Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks. Horticulturae, 7(11), 447.es
dc.identifier.issn2311-7524
dc.identifier.urihttp://hdl.handle.net/20.500.11939/7842
dc.description.abstractClimate change and global warming are leading to a change in weather patterns toward hot and cold waves. Citrus fruits are a tropical or subtropical crop whose growth is altered by changes in weather patterns. Thus, in the present work, two experiments are evaluated to obtain a screening method to select citrus rootstocks that help us to select new low-temperature-tolerant plant materials. One cold experiment was carried out with the Poncirus trifoliata and Citrus macrophylla rootstocks at 4 °C for 4, 8, 24 and 56 h. A second experiment was performed at 4 °C for 5 days with subsequent acclimatization lasting 0, 5, 10 and 24 h. The expression of the cold response genes CAMTA1, CAMTA3, CAMTA5, CBF1, ICE1 and COR413 IM1 was quantified. The results showed that the best rootstock selection strategy was the second experiment, as a higher expression of the genes CAMTA3, CAMTA5, CBF1 and COR413 IM1 was seen in the tolerant genotype P. trifoliata. We quantified the gene expression of proline biosynthesis P5CS1, dOAT and the proline transporters PROT1 and PROT2; the concentration of the amino acid proline in leaves was also quantified. These results once again showed that the best experiment to differentiate between tolerant and sensitive rootstocks was the second experiment with acclimation time.es
dc.language.isoenes
dc.publisherMDPIes
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectBreeding programses
dc.titleGene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstockses
dc.typearticlees
dc.authorAddressprimo_amp@gva.eses
dc.entidadIVIACentro de Citricultura y Producción Vegetales
dc.identifier.doi10.3390/horticulturae7110447es
dc.identifier.urlhttps://www.mdpi.com/2311-7524/7/11/447es
dc.journal.issueNumber11es
dc.journal.titleHorticulturaees
dc.journal.volumeNumber7es
dc.page.final447es
dc.page.initial447es
dc.relation.projectIDThis study was cofunded by the Ministerio de Ciencia e Innovación (RTI2018-098379-RI00) and the European Union through the European Regional Development Fund (ERDF) of the Generalitat Valenciana 2014-2020 (IVIA-51913).es
dc.rights.accessRightsopenAccesses
dc.source.typeelectronicoes
dc.subject.agrisF30 Plant genetics and breedinges
dc.subject.agrisF40 Plant ecologyes
dc.subject.agrisF60 Plant physiology and biochemistryes
dc.subject.agrisH50 Miscellaneous plant disorderses
dc.subject.agrisU30 Research methodses
dc.subject.agrovocCitruses
dc.subject.agrovocRootstockses
dc.subject.agrovocCold stresses
dc.subject.agrovocAbiotic stresses
dc.subject.agrovocGene expressiones
dc.subject.agrovocClimate changees
dc.subject.agrovocCold tolerancees
dc.subject.agrovocPoncirus trifoliataes
dc.subject.agrovocCitrus macrophyllaes
dc.type.hasVersionpublishedVersiones


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