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dc.contributor.authorPérez-Román, Estela
dc.contributor.authorBorredá, Carles
dc.contributor.authorLópez-García, Antonio
dc.contributor.authorTalón, Manuel
dc.date.accessioned2021-12-01T15:14:18Z
dc.date.available2021-12-01T15:14:18Z
dc.date.issued2021es
dc.identifier.citationPerez‐Roman, E., Borreda, C., Lopez‐García Usach, A. & Talon, M. (2021). Single‐nucleotide mosaicism in citrus: Estimations of somatic mutation rates and total number of variants. The Plant Genome, published on-line in 19 November 2021, e20162es
dc.identifier.issn1940-3372 (online ISSN)
dc.identifier.urihttp://hdl.handle.net/20.500.11939/7803
dc.description.abstractMost of the hundreds of citrus varieties are derived from spontaneous mutations. We characterized the dynamics of single-nucleotide mosaicism in a 36-yr-old clementine (Citrus ×clementina hort. ex Tanaka) tree, a commercial citrus whose vegetative behavior is known in detail. Whole-genome sequencing identified 73 reliable somatic mutations, 48% of which were transitions from G/C to A/T, suggesting ultraviolet (UV) exposure as mutagen. The mutations accumulated in sectorized areas of the tree in a nested hierarchy determined by the branching pattern, although some variants detected in the basal parts were also found in the new growth and were fixed in some branches and leaves of much younger age. The estimate of mutation rates in our tree was 4.4 × 10−10 bp−1 yr−1, a rate in the range reported in other perennials. Assuming a perfect configuration and taking advantage of previous counts on the number of total leaves of typical clementine trees, these mutation determinations allowed to estimate for the first time the total number of variants present in a standard adult tree (1,500–5,000) and the somatic mutations generated in a typical leaf flush (0.92–1.19). From an evolutionary standpoint, the sectoral distribution of somatic mutations and the habit of periodic foliar renewal of long-lived plants appear to increase genetic heterogeneity and, therefore, the adaptive role of somatic mutations reducing the mutational load and providing fitness benefits.es
dc.language.isoenes
dc.publisherWileyes
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectGenetic mosaicismes
dc.subjectSingle-nucleotide polymorphismes
dc.subjectMutation ratees
dc.subjectClementine treeses
dc.titleSingle-nucleotide mosaicism in citrus: Estimations of somatic mutation rates and total number of variantses
dc.typearticlees
dc.authorAddresstalon_man@gva.eses
dc.entidadIVIACentro de Genómicaes
dc.identifier.doi10.1002/tpg2.20162es
dc.identifier.urlhttps://acsess.onlinelibrary.wiley.com/doi/10.1002/tpg2.20162es
dc.journal.issueNumberPublished on-line (19 November 2021)es
dc.journal.titleThe Plant Genomees
dc.page.finale20162es
dc.page.initiale20162es
dc.relation.projectIDMinisterio de Ciencia, Innovación yUniversidades, Grant/AwardNumber:RTI2018- 097790-R-100.es
dc.relation.projectIDGeneralitatValenciana, InstitutoValenciano de InvestigacionesAgrarias, Grant/AwardNumbers: 51915, 52002.es
dc.rights.accessRightsopenAccesses
dc.source.typeelectronicoes
dc.subject.agrisF30 Plant genetics and breedinges
dc.subject.agrisU30 Research methodses
dc.subject.agrovocCitruses
dc.subject.agrovocSomatic mutationes
dc.subject.agrovocChromosomeses
dc.subject.agrovocDNA replicationes
dc.subject.agrovocVariantses
dc.subject.agrovocGenomeses
dc.type.hasVersionpublishedVersiones


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