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dc.contributor.authorGlasa, Miroslav
dc.contributor.authorPredajna, Lukas
dc.contributor.authorSihelská, Nina
dc.contributor.authorSoltys, Katarína
dc.contributor.authorRuiz-García, Ana B.
dc.date.accessioned2021-06-08T13:05:59Z
dc.date.available2021-06-08T13:05:59Z
dc.date.issued2020es
dc.identifier.citationGlasa, M., Predajňa, L., Sihelská, N., Šoltys, K., & Ruiz-García, A. B. (2020). Analysis of Virome by High-Throughput Sequencing Revealed Multiple Infection and Intra-Virus Diversity in a Single Grapevine Plant. Acta Horticulturae et Regiotecturae, 23(1), 35-39.es
dc.identifier.issn1335-2563 (printed edition)
dc.identifier.issn1338-5259 (online)
dc.identifier.urihttp://hdl.handle.net/20.500.11939/7412
dc.description.abstractThe ribosomal-depleted total RNA from white-berry grapevine (Vitis vinifera, SK933) plant showing severe chlorosis and downrolling of leaves was used for the high-throughput sequencing (HTS) analysis in order to unravel the potential contribution of the viral pathogens to the symptomatology observed. The combination of de novo assembly and mapping of ca. 1.1 millions of HTS reads enabled to identify and characterise a complex viral/viroid infection involving Grapevine leafroll-associated virus-2 (GLRaV-2), Grapevine leafroll-associated virus-3 (GLRaV-3), Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine rupestris vein feathering virus (GRVFV), Grapevine Syrah virus-1 (GSyV-1) and Hop stunt viroid (HSVd). The determined nearly complete genomes of GLRaV-2 SK933 showed its high genetic divergence from previously characterised isolates. In case of GRSPaV, two variants representing different evolutionary lineages have been identified in the plant. The results further pinpoint the complexity of grapevine viral diseases and show that mixed virus infection of grapevine is rather a rule than an exception.es
dc.language.isoenes
dc.publisherFaculty of Horticulture and Landscape Engineering/Slovak University of Agriculture in Nitra.es
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectDiversityes
dc.subjectNext generation sequencinges
dc.titleAnalysis of Virome by High-Throughput Sequencing Revealed Multiple Infection and Intra-Virus Diversity in a Single Grapevine Plantes
dc.typearticlees
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Protección Vegetal y Biotecnologíaes
dc.identifier.doi10.2478/ahr-2020-0009es
dc.identifier.urlhttps://www.sciendo.com/article/10.2478/ahr-2020-0009es
dc.journal.issueNumber1es
dc.journal.titleActa horticulturae et regiotecturaees
dc.journal.volumeNumber23es
dc.page.final39es
dc.page.initial35es
dc.relation.projectIDThis work was supported by the grant VEGA 2/0030/20 from the Scientific Grant Agency of the Ministry of Education and Slovak Academy of Sciences.es
dc.rights.accessRightsopenAccesses
dc.source.typeelectronicoes
dc.subject.agrisH20 Plant diseaseses
dc.subject.agrovocVitis viniferaes
dc.subject.agrovocViruseses
dc.type.hasVersionpublishedVersiones


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Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España