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dc.contributor.authorVentimilla, Daniel
dc.contributor.authorVelázquez, Karelia
dc.contributor.authorRuiz-Ruiz, Susana
dc.contributor.authorTerol, Javier
dc.contributor.authorPérez-Amador, Miguel A.
dc.contributor.authorVives, María C.
dc.contributor.authorGuerri, José
dc.contributor.authorTalón, Manuel
dc.contributor.authorTadeo, Francisco R.
dc.date.accessioned2021-05-26T10:26:15Z
dc.date.available2021-05-26T10:26:15Z
dc.date.issued2021es
dc.identifier.citationVentimilla, D., Velázquez, K., Ruiz-Ruiz, S., Terol, J., Pérez-Amador, M. A., Vives, M. C. et al (2021). IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)- like receptors regulate corolla abscission in Nicotiana benthamiana flowers. BMC Plant Biology, 21, 226.es
dc.identifier.issn1471-2229
dc.identifier.urihttp://hdl.handle.net/20.500.11939/7386
dc.description.abstractBackground: Abscission is an active, organized, and highly coordinated cell separation process enabling the detachment of aerial organs through the modification of cell-to-cell adhesion and breakdown of cell walls at specific sites on the plant body known as abscission zones. In Arabidopsis thaliana, abscission of floral organs and cauline leaves is regulated by the interaction of the hormonal peptide INFLORESCENCE DEFICIENT IN ABSCISSION (IDA), a pair of redundant receptor-like protein kinases, HAESA (HAE) and HAESA-LIKE2 (HSL2), and SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) co-receptors. However, the functionality of this abscission signaling module has not yet been demonstrated in other plant species. Results: The expression of the pair of NbenIDA1 homeologs and the receptor NbenHAE.1 was supressed at the base of the corolla tube by the inoculation of two virus-induced gene silencing (VIGS) constructs in Nicotiana benthamiana. These gene suppression events arrested corolla abscission but did not produce any obvious effect on plant growth. VIGS plants retained a higher number of corollas attached to the flowers than control plants, an observation related to a greater corolla breakstrength. The arrest of corolla abscission was associated with the preservation of the parenchyma tissue at the base of the corolla tube that, in contrast, was virtually collapsed in normal corollas. In contrast, the inoculation of a viral vector construct that increased the expression of NbenIDA1A at the base of the corolla tube negatively affected the growth of the inoculated plants accelerating the timing of both corolla senescence and abscission. However, the heterologous ectopic overexpression of citrus CitIDA3 and Arabidopsis AtIDA in N. benthamiana did not alter the standard plant phenotype suggesting that the proteolytic processing machinery was unable to yield active peptides. Conclusion: Here, we demonstrate that the pair of NbenIDA1 homeologs encoding small peptides of the IDA-like family and the receptor NbenHAE.1 control cellular breakdown at the base of the corolla tube awhere an adventitious AZ should be formed and, therefore, corolla abscission in N. benthamiana flowers. Altogether, our results provide the first evidence supporting the notion that the IDA-HAE/HSL2 signaling module is conserved in angiosperms.es
dc.language.isoenes
dc.publisherBMCes
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectAbscission Zonees
dc.subjectAbscission signaling modulees
dc.subjectCell separationes
dc.subjectGene silencing and overexpressiones
dc.subjectHormone peptidees
dc.subjectLRR-RLKses
dc.titleIDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowerses
dc.typearticlees
dc.authorAddresstadeo_fra@gva.eses
dc.entidadIVIACentro de Genómicaes
dc.identifier.doi10.1186/s12870-021-02994-8es
dc.identifier.urlhttps://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-021-02994-8es
dc.journal.issueNumber21es
dc.journal.titleBMC Plant Biologyes
dc.page.final226es
dc.page.initial226es
dc.relation.projectIDFinancial support was provided by the Ministerio de Ciencia, Innovación y Universidades (Spain) trough grant # RTI2018-097790-R-100 and from the Instituto Valenciano de Investigaciones Agrarias (Spain), through grants 51915 and 52002.es
dc.rights.accessRightsopenAccesses
dc.source.typeelectronicoes
dc.subject.agrisF60 Plant physiology and biochemistryes
dc.subject.agrisF63 Plant physiology - Reproductiones
dc.subject.agrisH20 Plant diseaseses
dc.subject.agrovocSolanaceaees
dc.type.hasVersionpublishedVersiones


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