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dc.contributor.authorViudes-De-Castro, María P.
dc.contributor.authorMarco-Jiménez, Francisco
dc.contributor.authorVicente, José Salvador
dc.contributor.authorMarín, Clara
dc.date.accessioned2021-05-13T06:32:40Z
dc.date.available2021-05-13T06:32:40Z
dc.date.issued2021es
dc.identifier.citationViudes-de-Castro, M.P., Marco-Jimenez, F., Vicente, J.S. & Marin, C. (2021). Antibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibiotics.,11(4), 1178.es
dc.identifier.issn2076-2615
dc.identifier.urihttp://hdl.handle.net/20.500.11939/7350
dc.description.abstractAlthough great attention is paid to hygiene during semen collection and processing, bacteria are commonly found in the semen of healthy fertile males of different species. As the storage of extended semen might facilitate bacterial growth, extenders are commonly supplemented with antibiotics. This study aimed to evaluate the antibacterial activity of ethylenediaminetetraacetic acid (EDTA), bestatin and chitosan-based nanoparticles added to rabbit semen extender and their effect on reproductive performance under field conditions. Four different extenders were tested, supplemented with antibiotics (TCG+AB), with EDTA and bestatin (EB), with EDTA, bestatin and chitosan-based nanoparticles (QEB) or without antibiotics (TCG-AB). Extended semen was cooled at 15 °C for three days. Cooled samples were examined for bacterial growth and semen quality every 24 h for 3 days. The enterobacteria count increased considerably during storage at 72 h in semen extended with TCG+AB and TCG-AB, while extenders EB and QEB showed a bacteriostatic effect over time. After 24, 48 and 72 h, quality characteristics were retained in all groups, with no significant motility differences, either in acrosome integrity, membrane functionality or the viability of spermatozoa. Additionally, bacterial concentration present in fresh semen did not affect reproductive performance. In conclusion, EDTA and bestatin exerted a potent bacteriostatic effect over time and could be used as an alternative to conventional antibiotics in rabbit semen extenderses
dc.language.isoenes
dc.publisherMDPIes
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectAntibacterial activityes
dc.subjectSemen qualityes
dc.titleAntibacterial Activity of Some Molecules Added to Rabbit Semen Extender as Alternative to Antibioticses
dc.typearticlees
dc.authorAddressviudes_mar@gva.eses
dc.entidadIVIACentro de Tecnología Animales
dc.identifier.doi10.3390/ani11041178es
dc.identifier.urlhttps://www.mdpi.com/2076-2615/11/4/1178es
dc.journal.issueNumber4es
dc.journal.titleAnimalses
dc.journal.volumeNumber11es
dc.page.final1178es
dc.page.initial1178es
dc.relation.projectIDFunding from the Ministry of Economy, Industry and Competitiveness (Research project: AGL2017-85162-C2-1-R)es
dc.rights.accessRightsopenAccesses
dc.source.typeelectronicoes
dc.subject.agrisL73 Animal diseaseses
dc.subject.agrisL70 Veterinary science and hygienees
dc.subject.agrovocArtificial inseminationes
dc.subject.agrovocAntibioticses
dc.subject.agrovocReproductive performancees
dc.type.hasVersionpublishedVersiones


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Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España