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dc.contributor.authorDomingo-Calap, María L.
dc.contributor.authorChase, Ornela
dc.contributor.authorEstapé, Mariona
dc.contributor.authorMoreno, Ana Beatriz
dc.contributor.authorLópez-Moya, Juan J.
dc.date.accessioned2021-05-07T13:25:14Z
dc.date.available2021-05-07T13:25:14Z
dc.date.issued2021es
dc.identifier.citationDomingo-Calap, M. L., Chase, O., Estapé, M., Moreno, A. B., & López-Moya, J. J. (2021). The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus. Frontiers in Microbiology, 12, 622.es
dc.identifier.issn1664-302X (e-ISSN)
dc.identifier.urihttp://hdl.handle.net/20.500.11939/7334
dc.description.abstractMixed viral infections in plants involving a potyvirus and other unrelated virus often result in synergistic effects, with significant increases in accumulation of the non-potyvirus partner, as in the case of melon plants infected by the potyvirus Watermelon mosaic virus (WMV) and the crinivirus Cucurbit yellow stunting disorder virus (CYSDV). To further explore the synergistic interaction between these two viruses, the activity of RNA silencing suppressors (RSSs) was addressed in transiently co-expressed combinations of heterologous viral products in Nicotiana benthamiana leaves. While the strong RSS activity of WMV Helper Component Proteinase (HCPro) was unaltered, including no evident additive effects observed when co-expressed with the weaker CYSDV P25, an unexpected negative effect of WMV P1 was found on the RSS activity of P25. Analysis of protein expression during the assays showed that the amount of P25 was not reduced when co-expressed with P1. The detrimental action of P1 on the activity of P25 was dose-dependent, and the subcellular localization of fluorescently labeled variants of P1 and P25 when transiently co-expressed showed coincidences both in nucleus and cytoplasm. Also, immunoprecipitation experiments showed interaction of tagged versions of the two proteins. This novel interaction, not previously described in other combinations of potyviruses and criniviruses, might play a role in modulating the complexities of the response to multiple viral infections in susceptible plants.es
dc.language.isoenes
dc.publisherFrontiers Mediaes
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectWatermelon mosaic viruses
dc.subjectCucurbit yellow stunting disorder viruses
dc.titleThe P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder viruses
dc.typearticlees
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Citricultura y Producción Vegetales
dc.identifier.doi10.3389/fmicb.2021.645530es
dc.identifier.urlhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.645530/fulles
dc.journal.issueNumber12es
dc.journal.titleFrontiers in Microbiologyes
dc.page.final622es
dc.page.initial622es
dc.relation.projectIDWork in the author’s laboratory was financed by grants AGL2016-75529-R and PID2019-105692RB-100 from Spanish Ministry of Science and Innovationes
dc.relation.projectIDMD-C was supported by FPI contract BES-2014-068970 from Ministry of Science and Innovation and FEDERes
dc.relation.projectIDOC was supported by 2018 FI_B 00329 from AGAUR, Generalitat de Catalunya, and Fons Social Europeues
dc.relation.projectIDAM was supported by European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 657527es
dc.relation.projectIDWe acknowledge financial support from the Spanish Ministry of Science and Innovation-State Research Agency (AEI), through the “Severo Ochoa Programme for Centres of Excellence in R&D” SEV-2015-0533 and CEX2019-000902-S, and from Generalitat de Catalunya CERCA Institution and support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).es
dc.rights.accessRightsopenAccesses
dc.source.typeelectronicoes
dc.subject.agrisH20 Plant diseaseses
dc.subject.agrisF30 Plant genetics and breedinges
dc.subject.agrisF60 Plant physiology and biochemistryes
dc.subject.agrovocWatermelonses
dc.subject.agrovocPotyviruseses
dc.subject.agrovocPlant viruseses
dc.type.hasVersionpublishedVersiones


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