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dc.contributor.authorDe-Ramón-Carbonell, Marta
dc.contributor.authorSánchez-Torres, Paloma
dc.date.accessioned2021-02-08T09:01:17Z
dc.date.available2021-02-08T09:01:17Z
dc.date.issued2021es
dc.identifier.citationde Ramón-Carbonell, M., & Sánchez-Torres, P. (2021). Penicillium digitatum MFS transporters can display different roles during pathogen-fruit interaction. International Journal of Food Microbiology, 337, 108918.es
dc.identifier.issn0168-1605
dc.identifier.urihttp://hdl.handle.net/20.500.11939/7077
dc.description.abstractMajor facilitator superfamily (MFS) comprises a large family of fungal transporters. In this work four Penicillium digitatum MFS transporters named PdMFS2–5 were identified and functionally characterized through gene elimination and gene overexpression with aim of unveil the similarities and differences among members of the same family during pathogen-fruit interaction. Fungal mutants in which each of the MFS transporters were individually deleted, displayed a clear effect on their infective capacity during citrus fruit infection especially in two of them. In contrast, the observed effect on fungicide sensitivity limits PdMFS2 and PdMFS3 as transporters underlying fungicide resistance. Moreover, overexpression transformants confirmed P. digitatum MFS transporters function and PdMFS2 and PdMFS3 were able to confer fungicide resistance to P. digitatum strains originally fungicide sensitive. Gene transcription rate depended on each MFS transporter being PdMFS4 the one with higher gene expression. Transcriptional profiling was similar regardless the P. digitatum strain. The gene expression analysis showed an increase of PdMFSs transcription in all overexpression transformants, particularly in Pd27 strain. Expression analysis carried out during P. digitatum-citrus fruit interaction confirmed the contribution of all PdMFSs, excepting PdMFS5, in fungal virulence. These results indicate that MFS fungal transporters might be part of different processes and can replace other genes functions giving them a very high degree of versatility.es
dc.language.isoenes
dc.publisherElsevieres
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectNutrients uptakees
dc.subjectFungicide sensitivityes
dc.subjectPostharvestes
dc.subjectSecondary transporterses
dc.titlePenicillium digitatum MFS transporters can display different roles during pathogen-fruit interactiones
dc.typearticlees
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Protección Vegetal y Biotecnologíaes
dc.identifier.doi10.1016/j.ijfoodmicro.2020.108918es
dc.identifier.urlhttps://www.sciencedirect.com/science/article/abs/pii/S0168160520304128es
dc.journal.issueNumber337es
dc.journal.titleInternational Journal of Food Microbiologyes
dc.page.final108918es
dc.page.initial108918es
dc.rights.accessRightsclosedAccesses
dc.source.typeelectronicoes
dc.subject.agrisH20 Plant diseaseses
dc.subject.agrovocCitruses
dc.subject.agrovocVirulencees
dc.type.hasVersionpublishedVersiones


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Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España