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dc.contributor.authorGorris, María T.
dc.contributor.authorSanz, Antonio
dc.contributor.authorPenyalver, Javier
dc.contributor.authorLópez, María M.
dc.contributor.authorColomer, Mario
dc.contributor.authorMarco-Noales, Ester
dc.date.accessioned2021-01-14T08:58:56Z
dc.date.available2021-01-14T08:58:56Z
dc.date.issued2021es
dc.identifier.urihttp://hdl.handle.net/20.500.11939/6967
dc.description.abstractMonoclonal antibodies (MAb) specific to Xylella fastidiosa were obtained through hybridoma technology using heat-treated somatic O antigens from LMG 17159strain. Ten stable hybrydoma clones secreting MAb were selected and their isotype was determined. The MAbs 2G1/PPD, IgG1 showed specificity for X. fastidiosa, detecting all the analyzed strains representing different subspecies, STs and hosts. Polyclonal antibodies (PAb) against X. fastidiosa were also produced and antiserum 17159-O/IVIA was selected for the highest titre and its excellent detection capability. MAb 2G1/PPD was tested against strain IVIA 5235 in PBS and in spiked raw extract samples from almond, olive, citrus, and other hosts and its sensitivity by DAS-ELISA was 104 CFU mL−1. The MAb also reacted with high affinity and avidity against X. fastidiosa by DASI-ELISA and Tissue print-ELISA. The diagnostic parameters of DAS-ELISA based on MAb were calculated and compared with the gold standard real-time PCR. The diagnostic specificity of MAb2G1/PPD was 100%, the diagnostic sensitivity was 88.5% compared to Harper’s real-time PCR and 89.9% compared to Francis’ real-time PCR. The agreement between the techniques was almost perfect according to the estimated Cohen’s kappa-index, even in symptomless almond trees. The developed immunological techniques represent sustainable and low-cost analysis tools, based on specific, homogeneous, and well-characterized MAbs, which can be obtained in unlimited quantities in a reproducible way and constitute a guarantee for the standardization of commercial kits. They are a valuable option within a polyphasic strategy for the detection of X. fastidiosa.es
dc.language.isoenes
dc.publisherMDPIes
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectDAS-ELISAes
dc.subjectDASI-ELISAes
dc.subjectTissue print-ELISAes
dc.subjectDiagnostic parameterses
dc.subjectCohen’s kappa indexes
dc.subjectSustainable diagnosises
dc.titleDetection and Diagnosis of Xylella fastidiosa by Specific Monoclonal Antibodieses
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Protección Vegetal y Biotecnologíaes
dc.identifier.doi10.3390/agronomy11010048es
dc.identifier.urlhttps://www.mdpi.com/2073-4395/11/1/48es
dc.journal.issueNumber1es
dc.journal.titleAgronomyes
dc.journal.volumeNumber11es
dc.page.final61es
dc.page.initial48es
dc.rights.accessRightsopenAccesses
dc.source.typeelectronicoes
dc.subject.agrisH20 Plant diseaseses
dc.subject.agrovocELISAes
dc.subject.agrovocMonoclonal antibodieses
dc.type.hasVersionpublishedVersiones


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Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España