Molecular Characterization of Olive Cultivars Using RAPD Markers
Date
2001Cita bibliográfica
Sanz-Cortés, F., Badenes, M. L., Paz, S., Iniguez, A., & Llacer, G. (2001). Molecular characterization of olive cultivars using RAPD markers. Journal of the American Society for Horticultural Science, 126(1), 7-12.Abstract
Forty olive (Olea europaea L.) cultivars from Valencia, Spain, were screened using random amplifiedpolymorphic
DNA (RAPD) markers. Eighteen selected decamer primers produced 34 reproducible amplification
fragments that were then used as polymorphic markers. The resulting combinations of these RAPD markers were used
to discriminate 40 cultivars. Results were analyzed for similarity among cultivars and the relatedness of polymorphisms
obtained between cultivars agreed with previous results using isozymes. Unweighted pair group method cluster analysis
of their similarity values revealed two main groups divided according to geographic origin within Valencia. A third group,
which included two Spanish cultivars from regions outside of Valencia, was clustered separately from the Valencian
cultivars. RAPD technology proved useful in discriminating closely related cultivars. There was no apparent clustering
of cultivars by fruit size or other morphological traits.