Inheritance in doubled-diploid clementine and comparative study with SDR unreduced gametes of diploid clementine
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Aleza, P., Cuenca, J., Juárez, J., Navarro, L., & Ollitrault, P. (2016). Inheritance in doubled-diploid clementine and comparative study with SDR unreduced gametes of diploid clementine. Plant Cell Reports, 35(8), 1573-1586.Abstract
Triploid breeding is developed worldwide to
create new seedless cultivars. Citrus triploid hybrids can be
recovered from 2x 9 2x sexual hybridizations as a consequence
of the formation of unreduced gametes (2n), or
from 4x 9 2x interploid hybridizations in which tetraploid
parents used are most often doubled-diploid (DD). Here we
have analyzed the inheritance in doubled-diploid clementine
and compared the genetic structures of gametes of DD
clementine with SDR unreduced gametes of diploid
clementine. Parental heterozygosity restitution (PHR) with
DD parents depends on the rate of preferential chromosome
pairing and thus the proportion of disomic versus
tetrasomic segregations. Doubled-diploid clementine largely
exhibited tetrasomic segregation. However, three
linkage groups had intermediate segregation and one had a
tendency for disomy. Significant doubled reduction rates
(DR) rates were observed in six of the nine LGs. Differences
of PHR between 2n SDR and 2x DD gametes were
highest in the centromeric region and progressively
decreased toward the distal regions where they were not
significant. Over all markers, PHR was lower (two-thirds)
in SDR 2n gametes than in DD-derived diploid gametes.
The two strategies appear complementary in terms of
genotypic variability. Interploid 4x 9 2x hybridization is
potentially more efficient for developing new cultivars that
are phenotypically closer to the diploid parent of the DD
than sexual hybridization through SDR 2n gametes. Conversely,
2x 9 2x triploidisation has the potential to produce
novel products with characteristics for market
segmentation strategies.