Molecular Assisted Selection for Pollination-Constant and Non-Astringent Type without Male Flowers in Spanish Germplasm for Persimmon Breeding
Date
2020Cita bibliográfica
Blasco, M., Gil-Muñoz, F., Naval, M. D. M., & Badenes, M. L. (2020). Molecular Assisted Selection for Pollination-Constant and Non-Astringent Type without Male Flowers in Spanish Germplasm for Persimmon Breeding. Agronomy, 10(8), 1172.Abstract
Persimmon (Diospyros kaki Thunb) species is a hexaploid genotype that has a morphologically
polygamous gyonodioecious sexual system. D. kaki bears unisexual flowers. The presence of male
flowers resulted in the presence of seeds in the varieties. The fruits of persimmon are classified
according to their astringency and the pollination events that produced seeds and modify the levels of
astringency in the fruit. The presence of seeds in astringent varieties as pollination variant astringent
(PVA), pollination variant non-astringent (PVNA) and pollination constant astringent (PCA) resulted
in fruits not marketable. Molecular markers that allow selection of the varieties according to the type
of flowers at the plantlet stage would allow selection of seedless varieties. In this study, a marker
developed in D. lotus by bulk segregant analysis (BSA) and amplified fragment length polymorphism
(AFLP) markers, named DlSx-AF4, has been validated in a germplasm collection of persimmon,
results obtained agree with the phenotype data. A second important trait in persimmon is the
presence of astringency in ripened fruits. Fruits non-astringent at the ripen stage named pollination
constant non-astringent (PCNA) are the objective of many breeding programs as they do not need
removal of the astringency by a postharvest treatment. Astringency in the hexaploid persimmon is a
dominant trait. The presence of at least one astringent allele confers astringency to the fruit. In this
paper we checked the marker developed linked to the AST gene. Our goal has been to validate both
markers in germplasm from different origins and to test the usefulness in a breeding program.