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dc.contributor.authorGlasa, Miroslav
dc.contributor.authorSoltys, Katarína
dc.contributor.authorPredajna, Lukás
dc.contributor.authorSihelská, Nina
dc.contributor.authorBudis, Jaroslav
dc.contributor.authorMrkvová, Michaela
dc.contributor.authorKraic, Ján
dc.contributor.authorMihálik, Daniel
dc.contributor.authorRuiz-García, Ana Belén
dc.date.accessioned2020-05-27T08:55:52Z
dc.date.available2020-05-27T08:55:52Z
dc.date.issued2019
dc.identifier.citationGlasa, M., Šoltys, K., Predajňa, L., Sihelská, N., Budiš, J., Mrkvová, M., ... & Ruiz-García, A. B. (2019). High-throughput sequencing of Potato virus M from tomato in Slovakia reveals a divergent variant of the virus. Plant Protection Science, 55(3), 159-166.
dc.identifier.issn1212-2580
dc.identifier.urihttp://hdl.handle.net/20.500.11939/6471
dc.description.abstractHigh-throughput sequencing (HTS) analysis of tomato (Solanum lycopersicum) samples revealed the presence of Potato virus M (PVM) in this crop in Slovakia. Full-length genomes of three PVM isolates were obtained using both HTS and Sanger sequencing validation. While two isolates (T40 and T50) were shown to belong to major Group I, a divergent T20 isolate was phylogenetically unrelated to any known PVM variant, potentially representing a new phylogenetic group. Despite a relatively high intraspecies diversity (17.3 ± 0.3%), no evidence of recombination was detected in the dataset of available complete PVM sequences. Conventional screening of tomato plants in Slovakia using ELISA and RT-PCR further confirmed a frequent occurrence of PVM in this host. Developed RT-PCR showed its polyvalence to detect the PVM Group I isolates, however, in silico analysis of primer binding sites indicated its compromised use for Group II isolates. Our results further pinpoint the significance of HTS for unbiased unveiling of virus diversity and a need for continual optimisation of molecular detection tools.es
dc.language.isoenes
dc.publisherCzech Academy of Agricultural Scienceses
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectPotato Virus Mes
dc.subjectFull-length genomees
dc.subjectRT-PCRes
dc.subjectPhylogenetical diversityes
dc.titleHigh-throughput sequencing of Potato virus M from tomato in Slovakia reveals a divergent variant of the viruses
dc.typearticlees
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias, Carretera CV-315, Km. 10,7 - 46113 Moncada (València)es
dc.entidadIVIACentro de Protección Vegetal y Biotecnologíaes
dc.identifier.doi10.17221/144/2018-PPSes
dc.identifier.urlhttps://www.agriculturejournals.cz/web/pps.htm?type=article&id=144_2018-PPSes
dc.journal.issueNumber3es
dc.journal.titlePlant Protection Sciencees
dc.journal.volumeNumber55es
dc.page.final166es
dc.page.initial159es
dc.source.typeelectronicoes
dc.subject.agrisH20 Plant diseaseses
dc.subject.agrovocSolanum lycopersicumes
dc.subject.agrovocCarlaviruseses
dc.type.hasVersionpublishedVersiones


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