Cooling of goat buck sperm in refrigerated bath or in itinere: effects on in vitro sperm quality
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AuthorMocé, Eva; Lozano-Palazón, S.A.; López, I.; Martínez-Granell, María M.; Bernacer, José; Vicente, C.; Gómez, Ernesto A.
Cita bibliográficaMoce, E., Lozano-Palazon, S. A., Lopez, I., Martinez-Granell, M., Bernacer, J., Vicente, C., & Gomez, E. A. (2019, October). Cooling of goat buck sperm in refrigerated bath or in itinere: effects on in vitro sperm quality. In Reproduction in Domestic Animals (54), pp. 106-107.
Goat buck sperm doses are kept at 4°C in refrigerated form. As a cold‐shock sensitive species, chilling to 4°C must be slow, which delays the doses delivery and their insemination. However, the time of transportation could be used for chilling. We studied if the sperm quality was similar between a system developed for chilling the doses during transportation and the programmable water bath (WB) in the lab. Twelve Murciano‐Granadina bucks were used. The concentration in each ejaculate (n = 12) was adjusted to 560 × 106 sperm/ml with skimmed milk‐glucose (0.2%; w:v) and semen was loaded into 0.25 ml plastic straws that were split into two treatments: half were chilled in a WB (in 90 min; theoretical cooling rate: −0.18°C/min) and the other half in a cooler (C; during 3 h 45 min; average cooling rate: −0.09°C/min). Total motile (%TM) and progressively motile (%PM) sperm were evaluated with a CASA system and live sperm (%LS) were evaluated with SYBR14/propidium iodide in a flow cytometer, according to the protocols from Konyali et al. (2013. Cryobiology, 67: 124–131). Fresh semen presented an average quality of 74.1% ± 2.2 TM, 49.4% ± 2.8 PM and 64.1% ± 2.9 LS. Straws chilled in WB and C exhibited similar TM (72.0% ± 4.3 and 76.3% ± 4.3) and PM sperm (50.5% ± 5.7 and 49.5% ± 5.7). However, straws chilled in C presented higher (p < 0.05) LS (67.8% ± 5.2) than samples chilled in WB (55.4% ± 5.2). In conclusion, straws chilled in the system adapted to the cooler exhibited similar percentages of motile but higher percentages of live sperm than samples chilled in the programmable water bath. Acknowledgements: AMURVAL, ACRIMUR and INIA RTA2017‐00049‐C02‐01 and FEDER funds.