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dc.contributor.authorDurán-Vila, Núria
dc.date.accessioned2020-02-21T11:51:31Z
dc.date.available2020-02-21T11:51:31Z
dc.date.issued2019
dc.identifier.citationDuran-Vila, N. (2019). Viroids as companions of a professional career. Viruses, 11(3), 245.
dc.identifier.issn1999-4915
dc.identifier.urihttp://hdl.handle.net/20.500.11939/6312
dc.description.abstractSince the early 1970s when "virus-like" agents were considered as the cause of two diseases (potato spindle tuber and citrus exocortis), their study and further characterization have been linked to the development and use of molecular biology tools. Sucrose density gradient centrifugation and polyacrylamide gel electrophoresis (PAGE) played a critical role in the pioneering studies of PSTVd and citrus exocortis viroid (CEVd). This was later modified by using other PAGEs (sequential PAGE, return PAGE, two-dimensional PAGE), and/or different staining methods (ethidium bromide, silver nitrate, etc.). Since then, disease-causing agents suspected to be viroids were usually subjected to a number of tests to define their: (i) Molecular nature (RNA or DNA; single stranded or double stranded; circular or linear RNA); (ii) molecular weight; (iii) secondary and tertiary structure. Further biological assays are also essential to establish the relationship of a viroid with plant disease and to fulfill Koch's postulates.
dc.language.isoen
dc.titleViroids as Companions of a Professional Career
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV- 315, Km. 10’7, 46113 Moncada, España.
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.3390/v11030245
dc.identifier.urlhttps://www.mdpi.com/1999-4915/11/3/245/htm
dc.journal.issueNumber3
dc.journal.titleViruses-Basel
dc.journal.volumeNumber11
dc.page.initial245
dc.rights.accessRightsopenAccess
dc.source.typeelectronico
dc.type.hasVersionpublishedVersion


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