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dc.contributor.authorDe-Ramón-Carbonell, Marta
dc.contributor.authorLópez-Pérez, Mario
dc.contributor.authorGonzalez-Candelas, Luis
dc.contributor.authorSánchez-Torres, Paloma
dc.date.accessioned2020-02-21T11:51:30Z
dc.date.available2020-02-21T11:51:30Z
dc.date.issued2019
dc.identifier.citationde Ramon-Carbonell, M., Lopez-Perez, M., Gonzalez-Candelas, L., & Sanchez-Torres, P. (2019). PdMFS1 Transporter Contributes to Penicilliun digitatum Fungicide Resistance and Fungal Virulence during Citrus Fruit Infection. Journal of Fungi, 5(4).
dc.identifier.issn2309-608X
dc.identifier.urihttp://hdl.handle.net/20.500.11939/6300
dc.description.abstractA new Penicillium digitatum major facilitator superfamily (MFS) transporter (PdMFS1) was identified and functionally characterized in order to shed more light on the mechanisms underlying fungicide resistance. PdMFS1 can play an important role in the intensification of resistance to fungicides normally used in P. digitatum postharvest treatments. In the PdMFS1 disrupted mutants, a slight effect in response to chemical fungicides was observed, but fungicide sensitivity was highly affected in the overexpression mutants which became resistant to wide range of chemical fungicides. Moreover, P. digitatum knock-out mutants exhibited a lower rate of fungal virulence when infected oranges were stored at 20 degrees C. Disease symptoms were higher in the PdMFS1 overexpression mutants coming from the low-virulent P. digitatum parental strain. In addition, the gene expression analysis showed an induction of PdMFS1 transcription in all overexpression mutants regardless from which progenitor came from, and four-time intensification of the parental wild type strain during citrus infection reinforcing PdMFS1 role in fungal virulence. The P. digitatum MFS transporter PdMFS1 contributes not only to the acquisition of wide range of fungicide resistance but also in fungal virulence during citrus infection.
dc.language.isoen
dc.titlePdMFS1 Transporter Contributes to Penicilliun digitatum Fungicide Resistance and Fungal Virulence during Citrus Fruit Infection
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.3390/jof5040100
dc.identifier.urlhttps://www.mdpi.com/2309-608X/5/4/100
dc.journal.issueNumber4
dc.journal.titleJournal Of Fungi
dc.journal.volumeNumber5
dc.page.initial100
dc.rights.accessRightsopenAccess
dc.source.typeelectronico
dc.type.hasVersionpublishedVersion


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