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dc.contributor.authorSalvador Vicente, Jose
dc.contributor.authorViudes-De-Castro, María P.
dc.contributor.authorIsai Cedano-Castro, Jose
dc.contributor.authorMarco-Jiménez, Francisco
dc.date.accessioned2019-05-15T10:37:43Z
dc.date.available2019-05-15T10:37:43Z
dc.date.issued2018es
dc.identifier.citationSalvador Vicente, J.; Pilar Viudes-de-Castro, M.; Isai Cedano-Castro, J.; Marco-Jimenez, F. (2018). Cryosurvival of rabbit embryos obtained after superovulation with corifollitropin alfa with or without LH. Animal Reproduction Science, 192, 321-327.es
dc.identifier.urihttp://hdl.handle.net/20.500.11939/6239
dc.description.abstractThe efficiency of an embryo bank depends on provision of optimal conditions for recovery, cryopreservation and transfer to a breed or strain. In this sense, increasing the number of embryos available using superovulation should improve the cryobank efficiency. However, vagueness of response to conventional protocols to control or increase ovarian response and the quality of oocytes and embryos and their cryotolerance remain a challenge. The aim of our study was to evaluate the effect of corifollitropin alpha (CTP) and a recombinant human FSH (rhFSH), alone or supplemented with rhLH, on embryo cryosurvival by in vitro development and OCT4 and NANOG mRNA abundance at blastocyst stage and offspring rate. In vitro development of vitrified embryos was not significantly affected by superstimulation with or without rhLH supplementation, resulting in similar development rates to those of the control groups (fresh and vitrified embryos from non-superstimulated donor does). Blastocysts developed from vitrified embryos showed higher levels of OCT4 transcript abundance than fresh control, while NANOG transcript abundance was only higher in the blastocysts developed from vitrified embryos after super stimulation treatment in comparison with control groups. The implantation and offspring rates at birth were negatively affected by supplementation with rhLH. Both rhFSH or CTP vitrified embryo groups showed an implantation rate similar to those of the control groups, but an offspring rate lower than control. In conclusion, embryos produced using corifollitropin alpha did not compromise the cryosurvival of vitrified embryos in the rabbit. In addition, this study points out the negative effect of rhLH supplementation in terms of offspring rate on embryo vitrification.es
dc.language.isoenes
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.titleCryosurvival of rabbit embryos obtained after superovulation with corifollitropin alfa with or without LHes
dc.typearticlees
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias, Carretera CV-315, Km. 10,7 - 46113 Moncada (Valencia)es
dc.entidadIVIACentro de Tecnología Animales
dc.identifier.doi10.1016/j.anireprosci.2018.03.034es
dc.journal.titleAnimal Reproduction Sciencees
dc.journal.volumeNumber192es
dc.page.final327es
dc.page.initial321es
dc.source.typeelectronicoes
dc.type.hasVersionacceptedVersion


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Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España