Insemination extender supplementation with bestatin and EDTA has no effect on rabbit reproductive performance
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AuthorCasares-Crespo, Lucía; Fernández-Serrano, Paula; Vicente, J. S.; Mocé, Eva; Castellini, C.; Stabile, A. M.; Viudes-De-Castro, María P.
Cita bibliográficaCasares-Crespo, L.; Fernandez-Serrano, P.; Vicente, J. S.; Moce, E.; Castellini, C.; Stabile, A. M.; Viudes-de-Castro, M. P. (2018). Insemination extender supplementation with bestatin and EDTA has no effect on rabbit reproductive performance. Theriogenology, 105, 61-65.
The addition of aminopeptidase inhibitors (AMIs) to rabbit semen extenders could be a solution to decrease the hormone degradation (GnRH) by the aminopeptidases existing in the seminal plasma. Therefore, the quantity of GnRH needed to induce ovulation in doe would be comparable with the amount administered intramuscularly (i.m.). This study was conducted to evaluate the effects of two AMIs (bestatin and EDTA) on rabbit semen quality parameters, beta nerve growth factor ((beta-NGF) degradation and reproductive performance after artificial insemination. Results showed that seminal quality was not affected by the incubation with AMIs; the values of motility, acrosome integrity and sperm viability were not significantly different between the AMIs and the control groups (positive i.m. and negative intravaginally without AMIs). In addition, the aminopeptidase activity of seminal plasma was inhibited in a 55.5% by the AMIs as well as beta-NGF degradation. On the other hand, regarding the effect of AMIs on reproductive performance, our results showed that the presence of bestatin and EDTA did neither affect fertility (85.3 vs. 88.6%), nor the prolificacy rate (10.12 vs. 10.51 kits per delivery), comparing AMIs group to positive control group, respectively. We conclude that the addition of specific AMIs in the rabbit semen extender has no effect on reproductive performance. Therefore, due to the fact that AMIs inhibit part of the aminopeptidase activity that degrades the GnRH analogue and beta-NGF, they could be used to develop new extenders with less hormone concentration. (C) 2017 Elsevier Inc. All rights reserved.