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dc.contributor.authorMorán, Félix
dc.contributor.authorOlmos, Antonio
dc.contributor.authorLotos, Leonidas
dc.contributor.authorKatis, Nikolaos
dc.contributor.authorGlasa, Miroslav
dc.contributor.authorPredajna, Lukas
dc.contributor.authorRuiz-García, Ana B.
dc.date.accessioned2018-12-11T08:15:00Z
dc.date.available2018-12-11T08:15:00Z
dc.date.issued2018es
dc.identifier.citationMorán F, Olmos A, Lotos L, Predajňa L, Katis N, Glasa M, et al. (2018) A novel specific duplex real-time RT-PCR method for absolute quantitation of Grapevine Pinot gris virus in plant material and single mites. PLoS ONE 13(5): e0197237. https://doi.org/10.1371/journal.pone.0197237es
dc.identifier.urihttp://hdl.handle.net/20.500.11939/6143
dc.description.abstractGrapevine Pinot gris virus (GPGV) is a widely distributed grapevine pathogen that has been associated to the grapevine leaf mottling and deformation disease. With the aim of better understanding the disease epidemiology and providing efficient control strategies a specific and quantitative duplex TaqMan real-time RT-PCR assay has been developed. This method has allowed reliable quantitation of the GPGV titer ranging from 30 up to 3 x 108 transcript copies, with a detection limit of 70 viral copies in plant material. The assay targets a grapevine internal control that reduces the occurrence of false negative results, thus increasing the diagnostic sensitivity of the technique. Viral isolates both associated and non-associated to symptoms from Greece, Slovakia and Spain have been successfully detected. The method has also been applied to the absolute quantitation of GPGV in its putative transmission vector Colomerus vitis. Moreover, the viral titer present in single mites has been determined. In addition, in the current study a new polymorphism in the GPGV genome responsible for a shorter movement protein has been found. A phylogenetic study based on this genomic region has shown a high variability among Spanish isolates and points to a different evolutionary origin of this new polymorphism. The methodology here developed opens new possibilities for basic and epidemiological studies as well as for the establishment of efficient control strategies.es
dc.language.isoenes
dc.publisherPLOS ONEes
dc.subjectDetection, vector, grapevine, quantification, variabilityes
dc.subjectTrichoviruses
dc.titleA novel specific duplex real-time RT-PCR method for absolute quantitation of Grapevine Pinot gris virus in plant material and single mitesen
dc.typearticlees
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.entidadIVIACentro de Protección Vegetal y Biotecnologíaes
dc.identifier.doi10.1371/journal.pone.0197237es
dc.identifier.urlhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0197237es
dc.journal.issueNumber5es
dc.journal.titlePLOS ONEes
dc.journal.volumeNumber13es
dc.page.final14es
dc.page.initial1es
dc.relation.projectIDINIA RTA-2014-0006, INIA E-RTA2017-00009, European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 734736 y COST Action FA1407es
dc.relation.projectIDINIA RTA-2014-0006, INIA E-RTA2017-00009, European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 734736 y COST Action FA1407es
dc.source.typeelectronicoes
dc.subject.agrisH20 Plant diseaseses
dc.subject.agrovocDisease preventiones
dc.subject.agrovocGrapevineses
dc.subject.agrovocTrichoviruses
dc.type.hasVersionpublishedVersion


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