Identification of a new polymorphism in the MP/CP region of Spanish isolates of Grapevine Pinot gris virus
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AuthorMorán, Félix; Predajňa; Katis, Nikolaos; Glasa, Miroslav; Ruiz-García, Ana Belén; Olmos, Antonio; Lotos, Leonidas
Cita bibliográficaMorán, F., Olmos, A., Lotos, L., Predajňa, L., Katis, N., Glasa, M., Maliogka, V., Ruiz-García, A. (2018) Identification of a new polymorphism in the MP/CP region of Spanish isolates of Grapevine Pinot gris virus. Póster presentado al XIX Congreso de la Sociedad Española de Fitopatología, Toledo, España.
Grapevine Pinot gris virus (GPGV) genome consists of one molecule of positive single stranded RNA that contains three overlapping open reading frames (ORFs) and two untranslated regions (5’ and 3’ UTRs). ORF1 encodes a methyltransferase, a helicase and the RNA dependent RNA polymerase (RdRp),ORF2 encodes the movement protein (MP) and ORF3 encodes the coat protein (CP). Several studies have reported the existence of high variability in GPGV genomes. Among this genomic diversity, a polymorphism involving a stop codon that produces a six amino acids shorter MP has been previously described. This polymorphism has been related to the symptoms observed in GPGV infected grapevines. In this study the genomic variability of MP/CP region, symptomatology and phylogenetic relationships of 12 Spanish isolates have been determined. A new polymorphism has been identified in the MP/CP region of some Spanish isolates. It involves the presence of a stop codon signal three nucleotides downstream from the polymorphism previously reported and would determine the synthesis of a five amino acids shorter MP. However, the phylogenetic study of these genome variants showed that the new polymorphism clusters in a different clade than the previously reported one, suggesting that these two polymorphisms, that result in a similar shortening of the GPGV MP, are independent evolutionary events. No association between the presence of these genetic variants and the symptomatology of Spanish GPGV infected grapevines has been found, suggesting that other factors would be involved in the epidemiology of the GPGV associated disease. These results give new insights in the study of GPGV genomic variability.