The sodium transporter encoded by the HKT1;2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity
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AuthorJaime-Perez, Noelia; Pineda, Benito; Garcia-Sogo, Begona; Atares, Alejandro; Athman, Asmini; Byrt, Caitlin S.; Olias, Raquel; Jose Asins, Maria; Gilliham, Matthew; Moreno, Vicente; Belver, Andres
Cita bibliográficaJaime-Perez, N., Pineda, B., Garcia-Sogo, B., Atares, A., Athman, A., Byrt, C. S., Olias, R., Jose Asins, M., Gilliham, M., Moreno, V., Belver, A. (2017). The sodium transporter encoded by the HKT1,2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity. Plant Cell and Environment, 40(5), 658-671.
Excessive soil salinity diminishes crop yield and quality. In a previous study in tomato, we identified two closely linked genes encoding HKT1-like transporters, HKT1;1 and HKT1;2, as candidate genes for a major quantitative trait locus (kc7.1) related to shoot Na+/K+ homeostasis - a major salt tolerance trait - using two populations of recombinant inbred lines (RILs). Here, we determine the effectiveness of these genes in conferring improved salt tolerance by using two near-isogenic lines (NILs) that were homozygous for either the Solanum lycopersicum allele (NIL17) or for the Solanum cheesmaniae allele (NIL14) at both HKT1 loci; transgenic lines derived from these NILs in which each HKT1;1 and HKT1;2 had been silenced by stable transformation were also used. Silencing of ScHKT1;2 and SlHKT1;2 altered the leaf Na+/K+ ratio and caused hypersensitivity to salinity in plants cultivated under transpiring conditions, whereas silencing SlHKT1;1/ScHKT1;1 had a lesser effect. These results indicate that HKT1;2 has the more significant role in Na+ homeostasis and salinity tolerance in tomato.