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dc.contributor.authorMoreno, A.
dc.contributor.authorBertolini, Edson
dc.contributor.authorOlmos, Antonio
dc.contributor.authorCambra, Mariano
dc.contributor.authorFereres, A.
dc.date.accessioned2017-06-01T10:12:50Z
dc.date.available2017-06-01T10:12:50Z
dc.date.issued2007
dc.identifier.citationMoreno, A., Bertolini, E., Olmos, A., Cambra, M., Fereres, A. (2007). Estimation of vector propensity for Lettuce mosaic virus based on viral detection in single aphids. Spanish Journal of Agricultural Research, 5(3), 376-384.
dc.identifier.issn1695-971X
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5689
dc.description.abstractLettuce mosaic virus (LMV) is transmitted by aphids nonpersistently causing severe disease outbreaks in commercial lettuce crops. New strategies to control plant viruses have arisen based on molecular techniques, which analyze plantvirus-vector interactions. In this work, two PCR-based methods with a previous immunocapture phase, have been developed to detect LMV in single aphids. Detection rates using a RT-nested-PCR method in single aphids and transmission efficiency of Myzus persicae (vector species) and Nasonovia ribisnigri (nonvector species) were compared. Although the percentage of viruliferous aphids for N. ribisnigri (45.8 +/- 2.3) was higher than for M. persicae (39.2 +/- 3.5) after the same acquisition access period, N. ribisnigri was unable to transmit the virus while M. persicae proved to be an efficient vector (with a transmission rate per single aphid of 10.4 +/- 0.8). A method was proposed to estimate vector propensity for nonpersistent viruses based on the relationship between the percentage of viruliferous aphids and their transmission ability. This methodology could be applied to decision-making and implementing control strategies to prevent virus spreading.
dc.language.isoen
dc.titleEstimation of vector propensity for Lettuce mosaic virus based on viral detection in single aphids
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias, Carretera CV-315, Km. 10,7 - 46113 Moncada (València)
dc.date.issuedFreeFormSEP 2007
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.journal.issueNumber3
dc.journal.titleSpanish Journal of Agricultural Research
dc.journal.volumeNumber5
dc.page.final384
dc.page.initial376
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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