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dc.contributor.authorMocé, Eva
dc.contributor.authorBlanch, Eva
dc.contributor.authorTalavan, Amparo
dc.contributor.authorViudes-De-Castro, María P.
dc.date.accessioned2017-06-01T10:12:43Z
dc.date.available2017-06-01T10:12:43Z
dc.date.issued2015
dc.identifier.citationMoce, E., Blanch, E., Talavan, A., Viudes-de-Castro, M.P. (2015). Effect of different freezing velocities on the quality and fertilising ability of cryopreserved rabbit spermatozoa. Reproduction Fertility and Development, 27(5), 846-851.
dc.identifier.issn1031-3613
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5637
dc.description.abstractThe freezing step of the cryopreservation protocol negatively influences the quality and fertilising ability of rabbit spermatozoa. This study determines the effect of different rates of freezing on the quality and fertilising ability of rabbit spermatozoa cryopreserved with dimethylsulfoxide (DMSO) (1.75M) and sucrose (0.05M). Ejaculates from meat rabbit line males (n=12) were pooled and each pool (n=7) was split into four aliquots. One group of straws (control, C) was frozen in static liquid nitrogen vapour (5cm above the liquid nitrogen, 10min) and the other groups were frozen at different freezing rates (degrees Cmin(-1)) from -6 degrees C to -100 degrees C using a programmable freezer: slow (-15 degrees Cmin(-1), S), medium (-40 degrees Cmin(-1), M) or fast (-60 degrees Cmin(-1), F). After thawing (50 degrees C, 12s), the quality was highest (P<0.05) in C and M samples and lowest in S and F samples. F samples presented the lowest litter sizes (P0.05) and fertility whilst M samples exhibited the highest values. In conclusion, the freezing rate affects both the quality and the fertilising ability of frozen-thawed rabbit spermatozoa, with both slow (-15 degrees Cmin(-1)) and fast (-60 degrees Cmin(-1)) freezing rates being detrimental for the quality and fertilising ability.
dc.language.isoen
dc.titleEffect of different freezing velocities on the quality and fertilising ability of cryopreserved rabbit spermatozoa
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeForm2015
dc.entidadIVIACentro de Tecnología Animal
dc.identifier.doi10.1071/RD14009
dc.journal.issueNumber5
dc.journal.titleReproduction Fertility and Development
dc.journal.volumeNumber27
dc.page.final851
dc.page.initial846
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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