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dc.contributor.authorMaserti, Bianca E.
dc.contributor.authorDel Carratore, R.
dc.contributor.authorDella Croce, C. M.
dc.contributor.authorPodda, A.
dc.contributor.authorMigheli, Q.
dc.contributor.authorFroelicher, Yann
dc.contributor.authorLuro, Francois
dc.contributor.authorMorillon, Raphael
dc.contributor.authorOllitrault, Patrick
dc.contributor.authorTalón, Manuel
dc.contributor.authorRossignol, M.
dc.date.accessioned2017-06-01T10:12:39Z
dc.date.available2017-06-01T10:12:39Z
dc.date.issued2011
dc.identifier.citationMaserti, B. E., Del Carratore, R., Della Croce, C. M., Podda, A., Migheli, Q., Froelicher, Y., Luro, F., Morillon, R., Ollitrault, P., Talón, M., Rossignol, M. (2011). Comparative analysis of proteome changes induced by the two spotted spider mite Tetranychus urticae and methyl jasmonate in citrus leaves. Journal of Plant Physiology, 168(4), 392-402.
dc.identifier.issn0176-1617
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5607
dc.description.abstractCitrus plants are currently facing biotic and abiotic stresses. Therefore, the characterization of molecular traits involved in the response mechanisms to stress could facilitate selection of resistant varieties. Although large cDNA microarray profiling has been generated in citrus tissues, the available protein expression data are scarce, in this study, to identify differentially expressed proteins in Citrus clementina leaves after infestation by the two-spotted spider mite Tetranychus urticae, a proteome comparison was undertaken using two-dimensional gel electrophoresis. The citrus leaf proteome profile was also compared with that of leaves treated over 0-72 h with methyl jasmonate, a compound playing a key role in the defense mechanisms of plants to insect/arthropod attack. Significant variations were observed for 110 protein spots after spider mite infestation and 67 protein spots after MeJA treatments. Of these, 50 proteins were successfully identified by liquid chromatography-mass spectrometry-tandem mass spectrometry. The majority constituted photosynthesis- and metabolism-related proteins. Five were oxidative stress associated enzymes, including phospholipid glutathione peroxidase, a salt stressed associated protein, ascorbate peroxidase and Mn-superoxide dismutase. Seven were defense-related proteins, such as the pathogenesis-related acidic chitinase, the protease inhibitor miraculin-like protein, and a lectin-like protein. This is the first report of differentially regulated proteins after T. urticae attack and exogenous MeJA application in citrus leaves. (C) 2010 Elsevier GmbH. All rights reserved.
dc.language.isoen
dc.titleComparative analysis of proteome changes induced by the two spotted spider mite Tetranychus urticae and methyl jasmonate in citrus leaves
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormMAR 1 2011
dc.entidadIVIACentro de Genómica
dc.identifier.doi10.1016/j.jplph.2010.07.026
dc.journal.abbreviatedTitleJ.Plant Physiol.
dc.journal.issueNumber4
dc.journal.titleJournal of Plant Physiology
dc.journal.volumeNumber168
dc.page.final402
dc.page.initial392
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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