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dc.contributor.authorMarti, Miguel
dc.contributor.authorTrotonda, Maria P.
dc.contributor.authorTormo-Mas, María A.
dc.contributor.authorVergara-Irigaray, Marta
dc.contributor.authorCheung, Ambrose L.
dc.contributor.authorLasa, Inigo
dc.contributor.authorPenadés, José R.
dc.identifier.citationMarti, Miguel, P. Trotonda, M., Angeles Tormo-Mas, M., Vergara-Irigaray, Marta, Cheung, Ambrose L., Lasa, Inigo, Penades, J.R. (2010). Extracellular proteases inhibit protein-dependent biofilm formation in Staphylococcus aureus. Microbes and Infection, 12(1), 55-64.
dc.description.abstractIn Staphylococcus aureus, biofilm formation can be associated with the production of surface-anchored proteins, including Bap, SasG, FnBPs or Spa. By mutational analysis, and using a model strain in which biofilm formation was Bap-dependent, we found that sigma(B) was essential for protein-dependent biofilm development. Non-polar mutations of sigma(B) in genetically unrelated S. aureus strains lowered the Bap expression and completely impaired biofilm development. Although Northern blot analysis showed a slight reduction in bap, transcription, we demonstrated that Aur and SspA, two proteases that are overexpressed in the sigB mutant strain and are capable of degrading Bap, inhibit biofilm formation. Interestingly, a double sigB-agr mutant, which showed a diminished capacity to express extracellular proteases, was able to restore biofilm formation. Since the vast majority of the S. aureus global regulators control the expression of the extracellular proteases, the results of this work demonstrate the existence of a new pathway controlling protein-mediated biofilm formation in which different global regulators modulate biofilm formation by controlling the expression of S. aureus extracellular proteases.
dc.titleExtracellular proteases inhibit protein-dependent biofilm formation in Staphylococcus aureus
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes 2010
dc.entidadIVIACentro de Tecnología Animal
dc.journal.titleMicrobes and Infection

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