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dc.contributor.authorAlioto, D.
dc.contributor.authorMalfitano, M.
dc.contributor.authorTroisi, A.
dc.contributor.authorPeluso, A.
dc.contributor.authorMartin, Susana
dc.contributor.authorMilne, R. G.
dc.contributor.authorGuerri, José
dc.contributor.authorMoreno, Pedro
dc.date.accessioned2017-06-01T10:12:34Z
dc.date.available2017-06-01T10:12:34Z
dc.date.issued2003
dc.identifier.citationAlioto, D., Malfitano, M., Troisi, A., Peluso, A., Martin, S., Milne, R. G., Guerri, J., Moreno, P. (2003). Variability of the coat protein gene of Citrus psorosis virus in Campania, southern Italy. Archives of Virology, 148(11), 2155-2166.
dc.identifier.issn0304-8608
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5565
dc.description.abstractVariability of the Coat protein (CP) gene of Citrus psorosis virus (CPsV) was assessed serologically, and by sequence analyses of two genomic regions located in the 3' (region C) and 5' (region V) halves of the gene. Analysis of 53 psorosis field sources from Campania, Italy, with 23 monoclonal antibodies revealed nine serogroups and at least ten different epitopes. Sequence analysis of 19 of these sources showed limited nucleotide diversity of the CP gene in the population. Diversity was slightly higher in region V than in region C. Phylogenetic analysis of the V and C regions of the CP showed that the Campania sources of CPsV were clearly separated from the CPsV-4 isolate from Florida. For C region, most of the CPsV sources clustered together, whereas two clusters were observed for region V. The ratio between nonsynonymous and synonymous substitutions for regions C (0.083) and V (0.345) indicated negative selective pressure for amino acid changes, more intense in the C region. No correlation was found between serogroups and specific aminoacid sequences, field location or citrus cultivar.
dc.language.isoen
dc.titleVariability of the coat protein gene of Citrus psorosis virus in Campania, southern Italy
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormNOV 2003
dc.entidadIVIACentro de Tecnología Post-recolección
dc.identifier.doi10.1007/s00705-003-0170-1
dc.journal.abbreviatedTitleArch.Virol.
dc.journal.issueNumber11
dc.journal.titleArchives of Virology
dc.journal.volumeNumber148
dc.page.final2166
dc.page.initial2155
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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