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dc.contributor.authorLopez, Carmelo
dc.contributor.authorCervera, Magdalena
dc.contributor.authorFagoaga, Carmen
dc.contributor.authorMoreno, Pedro
dc.contributor.authorNavarro, Luis
dc.contributor.authorFlores, Ricardo
dc.contributor.authorPena, Leandro
dc.date.accessioned2017-06-01T10:12:29Z
dc.date.available2017-06-01T10:12:29Z
dc.date.issued2010
dc.identifier.citationLopez, Carmelo, Cervera, M., Fagoaga, C., Moreno, P., Navarro, L., Flores, R., Pena, L. (2010). Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime. Molecular Plant Pathology, 11(1), 33-41.
dc.identifier.issn1464-6722
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5511
dc.description.abstractP>Mexican lime plants transformed with the 3'-terminal 549 nucleotides of the Citrus tristeza virus (CTV) genome in sense, antisense and intron-hairpin formats were analysed for transgene-derived transcript and short interfering RNA (siRNA) accumulation, and for CTV resistance. Propagations from all sense, antisense and empty-vector transgenic lines were susceptible to CTV, except for a single sense-line plant with a complex transgene integration pattern that showed transgene-derived siRNAs in association with low levels of the transgene-derived transcript. In contrast, nine of 30 intron-hairpin lines showed CTV resistance, with 9%-56% of bud-propagated plants, depending on the line, remaining uninfected on graft inoculation, and the others being susceptible. Although resistance was always associated with the presence of transgene-derived siRNAs, their level in different sense and intron-hairpin transformants was variable irrespective of the response to CTV infection. In intron-hairpin lines with single transgene integration, CTV resistance was correlated with low accumulation of the transgene-derived transcript rather than with high accumulation of transgene-derived siRNAs.
dc.language.isoen
dc.titleAccumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormJAN 2010
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1111/j.1364-3703.2009.00566.X
dc.journal.issueNumber1
dc.journal.titleMolecular Plant Pathology
dc.journal.volumeNumber11
dc.page.final41
dc.page.initial33
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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