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dc.contributor.authorLlop, Pablo
dc.contributor.authorMurillo, Jesús
dc.contributor.authorLastra, Beatriz
dc.contributor.authorLópez, María M.
dc.date.accessioned2017-06-01T10:12:28Z
dc.date.available2017-06-01T10:12:28Z
dc.date.issued2009
dc.identifier.citationLlop, P., Murillo, Jesus, Lastra, Beatriz, Lopez, M.M. (2009). Recovery of Nonpathogenic Mutant Bacteria from Tumors Caused by Several Agrobacterium tumefaciens Strains: a Frequent Event?. Applied and Environmental Microbiology, 75(20), 6504-6514.
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5504
dc.description.abstractWe have evaluated the interaction that bacterial genotypes and plant hosts have with the loss of pathogenicity in tumors, using seven Agrobacterium tumefaciens strains inoculated on 12 herbaceous and woody hosts. We performed a screening of the agrobacteria present inside the tumors, looking for nonpathogenic strains, and found a high variability of those strains in this niche. To verify the origin of the putative nonpathogenic mutant bacteria, we applied an efficient, reproducible, and specific randomly amplified polymorphic DNA analysis method. In contrast with previous studies, we recovered a very small percentage (0.01%) of nonpathogenic strains that can be considered true mutants. Of 5,419 agrobacterial isolates examined, 662 were nonpathogenic in tomato, although only 7 (from pepper and tomato tumors induced by two A. tumefaciens strains) could be considered to derive from the inoculated strain. Six mutants were affected in the transferred DNA (T-DNA) region; one of them contained IS426 inserted into the iaaM gene, whereas the whole T-DNA region was apparently deleted in three other mutants, and the virulence of the remaining two mutants was fully restored with the T-DNA genes as well. The plasmid profile was altered in six of the mutants, with changes in the size of the Ti plasmid or other plasmids and/or the acquisition of new plasmids. Our results also suggest that the frequent occurrence of nonpathogenic clones in the tumors is probably due to the preferential growth of nonpathogenic agrobacteria, of either endophytic or environmental origin, but different from the bacterial strain inducing the tumor.
dc.language.isoen
dc.titleRecovery of Nonpathogenic Mutant Bacteria from Tumors Caused by Several Agrobacterium tumefaciens Strains: a Frequent Event?
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormOCT 15 2009
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1128/AEM.01867-08
dc.journal.abbreviatedTitleAppl.Environ.Microbiol.
dc.journal.issueNumber20
dc.journal.titleApplied and Environmental Microbiology
dc.journal.volumeNumber75
dc.page.final6514
dc.page.initial6504
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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