Show simple item record

dc.contributor.authorJimenez-Trigos, E.
dc.contributor.authorVicente, J. S.
dc.contributor.authorMoce, Eva
dc.contributor.authorNaturil-Alfonso, C.
dc.contributor.authorFernandez-Gonzalez, R.
dc.contributor.authorGutiérrez-Adan, A.
dc.contributor.authorMarco-Jimenez, F.
dc.date.accessioned2017-06-01T10:12:20Z
dc.date.available2017-06-01T10:12:20Z
dc.date.issued2013
dc.identifier.citationJimenez-Trigos, E., Vicente, J.S., Moce, E., Naturil-Alfonso, C., Fernandez-Gonzalez, R., Gutierrez-Adan, A., Marco-Jimenez, F. (2013). Treatment with cholE.ol-loaded methyl-beta-cyclodextrin increased the cholE.ol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes. Cryobiology, 67(1), 106-108.
dc.identifier.issn0011-2240
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5413
dc.description.abstractMembrane cholesterol:phospholipids ratio is an important determinant of cell chilling sensitivity. At low temperatures, major membrane destabilisation occurs when the membrane undergoes a phase transition. To increase membrane fluidity and stability during cooling and thus increase oocyte cryoresistance, cholesterol has been added to the plasma membrane. This study was conducted to determine if cholesterol could be incorporated into rabbit oocytes by incubation with cholesterol-loaded methyl-beta-cyclodextrin (CLC) and if added cholesterol could improve the developmental ability of cryopreserved oocytes after parthenogenetic activation or intracytoplasmic sperm injection. Fresh, frozen and vitrified oocytes incubated with CLC containing 20% NBD-labelled cholesterol (NBD-CLC) were evaluated using confocal microscopy. Fluorescence intensity was higher in fresh oocytes than in cryopreserved ones. Pre-treating rabbit oocytes with 1 mg of NBD-CLC/mL did not improve cleavage and developmental rates after cryopreservation. Results showed that treatment with CLC increased the cytoplasmic cholesterol content, but did not improve cleavage rate and developmental competence of cryopreserved oocytes. (C) 2013 Elsevier Inc. All rights reserved.
dc.language.isoen
dc.titleTreatment with cholesterol-loaded methyl-beta-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes
dc.typearticle
dc.date.issuedFreeFormAUG 2013
dc.identifier.doi10.1016/j.cryobiol.2013.05.006
dc.journal.abbreviatedTitleCryobiology
dc.journal.issueNumber1
dc.journal.titleCryobiology
dc.journal.volumeNumber67
dc.page.final108
dc.page.initial106
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record